GEO DataSets

(Submitter supplied) We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

4 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔgalR to D39 wild type grown in M17 medium+ 0.5% (w/v) galactose (GalM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔgalR to D39 wild type grown in M17 medium+ 0.5% (w/v) glucose (GM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) In this study, we explore the impact of fucose on the transcriptome of S. pneumoniae D39. The expression of various genes and operons, including the fucose uptake PTS and utilization operon (fcs operon), was altered in the presence of fucose. By means of quantitative RT-PCR and β-galactosidase analysis, we demonstrate the role of the transcriptional regulator FcsR, present upstream of the fcs operon, as a transcriptional activator of the fcs operon. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparision of the Streptococcus pneumoniae D39 ccpA mutant to D39 wild-type grown in CDM + 0.5 % N-acetylglucosamine (NAG)

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL22631

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown at in CDM with 0.5% Glucose to grown in CDM with 0.5 % N-acetyleglucosamine (NAG)

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome Comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM with 0 mM to 10mM Niacin

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL22631

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔnaiR to D39 wild type grown in CDM

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL22631

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔcelR to D39 wild-type grown M17 medium + 0.5 % (w/v) Cellobiose (CM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

3 Samples

Download data: TXT

(Submitter supplied) The human pathogen Streptococcus pneumoniae has the ability to use the carbon- and energy source cellobiose due to the presence of a cellobiose-utilizing gene cluster (cel locus) in its genome. This system is regulated by the cellobiose-dependent transcriptional activator CelR, which has been previously shown to contribute to pneumococcal virulence. To get a broader understanding of the response of S. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

9 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium+ 0.5 % (w/v) Cellobiose (CM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17) hervasted at time point T2.

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

3 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium+ 0.5 % (w/v) Cellobiose (CM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17) hervasted at time point T1.

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

3 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔbguR to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

3 Samples

Download data: TXT

(Submitter supplied) In this study, we investigated the transcriptomic response of Streptococcus pneumoniae D39 to sialic acid (N-acetylneuraminic acid: Neu5Ac). Transcriptome comparison of the D39 wild-type grown in M17 medium with and without sialic acid revealed the elevated expression of various genes and operons including the nan gene cluster (nan operon-I and nanA gene). Our microarray analysis and promoter-lacZ fusion studies showed that the transcriptional regulator NanR acts as a transcriptional activator of nan operon-I and the nanA gene in the presence of sialic acid. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

8 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium toD39 wild-type grown in M17 medium + 0.5% sialic acid (SM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild-type grown in M17 medium + 0.5% sialic acid (SM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ΔnanR to D39 wild-type grown in M17 medium + 0.5% sialic acid (SM17).

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae D39 ∆ulaR to D39 wild-type grown in M17 medium + 10mM ascorbic acid (AM17)

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

2 Samples

Download data: TXT

(Submitter supplied) Transcriptome comparison of the Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose, revealed the elevated expression of various genes and operons, including the lac gene cluster that is organized into two operons i.e. lac operon-I (lacABCD) and lac operon-II (lacTFEG). Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster, revealed elevated expression of lac operon-I, even in the absence of lactose. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL11484

9 Samples

Download data: TXT