GEO DataSets

(Submitter supplied) Insulin resistance is central to diabetes and metabolic syndrome. To define the consequences of genetic insulin resistance distinct from those secondary to cellular differentiation or in vivo regulation, we generated induced pluripotent stem cells (iPSCs) from individuals with insulin receptor mutations and age-appropriate control subjects and studied insulin signaling and gene expression compared with the fibroblasts from which they were derived. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

17 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL13534 GPL571

79 Samples

Download data: CEL

(Submitter supplied) Skin fibroblasts from individuals with PBD-ZSD, a rare autosomal recessive disorder caused by peroxisome assembly defects, show defects in lipid metabolism that provide the basis for clinical diagnostic tests, but are not among the cell types most affected by disease. To explore phenotypes of more clinically relevant cell types, skin fibroblasts from PBD-ZSD patients and healthy controls were reprogrammed into iPS cells with all the hallmark properties of pluripotency. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

3 Samples

Download data: CEL

(Submitter supplied) Induced pluripotent stem (iPS) cells have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPS cells generated from different cell types are molecularly and functionally similar. Here, we show that iPS cells obtained from fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPS cells into embryoid bodies and different hematopoietic cells. Our results suggest that low-passage iPS cells retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and altered differentiation capacity. These observations might affect ongoing attempts to use iPS cells for disease modeling and also could be exploited for potential therapeutic applications to enhance differentiation into desired cell lineages. This series consists of triplicated mRNA expression microarray data (Affymetrix mouse gene ST 1.0) for an early passage (passage 4) of mouse iPS cells derived from bone marrow granulocytes, splenic B cells, tail tip fibroblasts, and skeletel muscle precursor cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3770

Platform:

GPL6246

12 Samples

Download data: CEL, CHP

(Submitter supplied) Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here, we show that iPSCs obtained from fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies (EBs) and different hematopoietic cells. more...

Organism:

Mus musculus

Type:

Methylation profiling by genome tiling array

Platform:

GPL10283

18 Samples

Download data: PAIR

(Submitter supplied) Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. We use expresson profiling to determine differnces in iPSCs with different cell of origin.

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS3771 GDS3772

Platforms:

GPL8321 GPL8759

45 Samples

Download data: CEL

Analysis of iPSCs (from p4 to p16) derived from different cell types (fibroblasts, hematopoietic, myogenic cells) through ectopic expression of transcription factors Oct4, Sox2, Klf4, cMyc. Results provide insight into the effect of passaging on iPSC functionality.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 4 cell type sets

Platform:

GPL8321

Series:

GSE22043

12 Samples

Download data: CEL

Analysis of iPSCs (at p10) derived from different cell types (fibroblasts, hematopoietic cells) through ectopic expression of transcription factors Oct4, Sox2, Klf4, cMyc. Results provide insight into the effect of passaging on iPSC functionality.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 5 cell type, 3 growth protocol sets

Platform:

GPL8759

Series:

GSE22043

33 Samples

Download data: CEL

Analysis of iPSCs (at p4) derived from different cell types (fibroblasts, hematopoietic, myogenic cells) through ectopic expression of transcription factors Oct4, Sox2, Klf4, cMyc. Results provide insight into the influence of cellular origin on transcriptional patterns of derivative iPSCs.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 4 cell type sets

Platform:

GPL6246

Series:

GSE22908

12 Samples

Download data: CEL, CHP

(Submitter supplied) We used a modified 5i/L/FA system to generate transgene-free naïve iPSCs directly from the fibroblasts of a patient suffering from β-thalassemia and further demonstrated efficient gene correction with a CRISPR/Cas9 system, which provides an improved strategy for personalized treatment of β-thalassemia.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL9052

14 Samples

Download data: TXT

(Submitter supplied) Gorlin syndrome is an autosomal dominant inherited syndrome that predisposes a patient to the formation of basal cell carcinomas, odontogenic keratocysts, and skeletal anomalies. Patched-1 (PTCH1) is a Hedgehog (Hh) receptor that acts as a negative regulator of constitutive Hh signaling by preventing the G protein-coupled receptor Smoothened from entering the cilium in the absence of Hh protein binding. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL24072

12 Samples

Download data: XLS

(Submitter supplied) Our recent approach to induction of pluripotent stem cells from fibroblasts using protein extract from mouse ESCs could overcome the potential tumorigenicity risks associated with random retroviral integration. Here we examine the epigenetic modifications and transcriptome in two kinds of iPSCs and a partially reprogrammed iPSC (iPSCp) generated from adult cardiac and skin fibroblasts independently to assess any perturbations of transcription program during reprogramming.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11002

14 Samples

Download data: BEDGRAPH

(Submitter supplied) Although not an affected cell type, skin fibroblasts from individuals with CC-ALD, an early onset X-linked neurological disorder, show defects in very long chain fatty acid (VLCFA) metabolism that provide the basis for clinical diagnostic tests. Skin fibroblasts from CC-ALD patients can be reprogrammed into iPS cells with all the hallmark properties of pluripotency. The iPS cell phenotypes may reflect the tissue-specificity of the lipid metabolic defects found in CC-ALD patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4451

Platform:

GPL571

18 Samples

Download data: CEL

(Submitter supplied) Although not an affected cell type, skin fibroblasts from individuals with childhood cerebral adrenoleukodystrophy (CCALD), an early onset X-linked neurological disorder, show defects in very long chain fatty acid (VLCFA) metabolism that provide the basis for clinical diagnostic tests. We report the gene expression profiles of fibroblasts from childhood cerebral adrenoleukodystrophy patients and healthy controls

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4559

Platform:

GPL570

10 Samples

Download data: CEL

Analysis of fibroblasts from patients with CCALD, the childhood cerebral form of X-linked adrenoleukodystrophy (X-ALD). X-ALD is an early onset X-linked neurological disorder affecting the nervous, adrenocortical, and male reproductive systems. Results provide insight into molecular basis of CCALD.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL570

Series:

GSE34308

10 Samples

Download data: CEL

Analysis of induced pluripotent stem cells (iPSC) derived from childhood cerebral adrenoleukodystrophy (CCALD) patient dermal fibroblasts. Inflammatory brain demyelination is observed in CCALD patients. Results provide insight into molecular mechanisms underlying CCALD pathogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 2 disease state sets

Platform:

GPL571

Series:

GSE34309

18 Samples

Download data: CEL

(Submitter supplied) RNA sequencing was performed to investigate ionizing radiation-dependent transcriptional change in human pluripotent cells and differentiated cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: DIFF

(Submitter supplied) About 25% of people within the general population are insulin resistant, increasing the risk for type 2 diabetes (T2D) and metabolic disease. Transcriptomic analysis of iPS cells differentiated into myoblasts (iMyos) from insulin resistant (I-Res) versus insulin sensitive (I-Sen) non-diabetic individuals reveals 306 genes increased and 271 genes decreased in expression in iMyos from insulin resistant donors with differences of 2-folds or more. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

16 Samples

Download data: CSV

(Submitter supplied) The difficulty associated with generating induced pluripotent stem cells (iPSC) from patients with chromosomal instability syndromes suggests the cellular DNA damage response poses a barrier to reprogramming. Here we demonstrate that fibroblasts from patients with ataxia-telangiectasia (A-T) can be reprogrammed to bona-fide iPSC, albeit at a reduced efficiency. A-T iPSC display defective radiation-induced signaling, radiosensitivity and cell cycle checkpoint defects. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

33 Samples

Download data: TXT

(Submitter supplied) Klinefelter’s Syndrome (KS) is one of the common chromosome aneuploidy diseases in males with unexplained physiological mechanism. iPSCs, are similar to ESCs in terms of indefinitive self-renewal and pluripotency, provided an alternative choice for modeling disease to facilitate the disease research in vitro. We used microarray to detect the global reprogramming of KS and normal fibroblast cells to iPSCs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

18 Samples

Download data: CEL