GEO DataSets

Analysis of wild type W303a cells across two cell cycles, a length of 2 hours after synchronization with alpha factor. Gene expression examined at 5-minute intervals.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 25 time sets

Platform:

GPL1914

Series:

GSE4987

50 Samples

Download data: GPR

(Submitter supplied) Transcription profiles across the yeast cell cycle. W303a cells were arrested with alpha factor in YEPD media. After release from arrest cells were sampled every 5 min for 2 hr. Keywords: cell cycle

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2350

Platform:

GPL1914

50 Samples

Download data: GPR

(Submitter supplied) In S. cerevisiae, the forkhead transcription factor Hcm1 has been involved in chromosome segregation, spindle pole dynamics and budding. We found that, in response to oxidative stress stimuli, Hcm1 shifts from cytoplasm to the nucleus, interacting with the histone deacetylase Sir2, which regulates Hcm1 localization. Hcm1-overexpressing cells showed increased resistance to oxidative stress which can be attributed to increased catalase and Sod2 activities. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL10039

12 Samples

Download data: GPR

(Submitter supplied) There are about 800 genes in Saccharomyces cerevisiae whose transcription is cell-cycle regulated. Some of these form clusters of co-regulated genes. The 'CLB2' cluster contains 33 genes whose transcription peaks early in mitosis, including CLB1, CLB2, SWI5, ACE2, CDC5, CDC20 and other genes important for mitosis. Here we find that the genes in this cluster lose their cell cycle regulation in a mutant that lacks two forkhead transcription factors, Fkh1 and Fkh2. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2650 GPL51

26 Samples

Download data

(Submitter supplied) Analysis of gene expression across the cell cycle from wild type cells, and cells expressing alleles of Yox1, Yhp1, Hcm1, and Tos4 that cannot be phosphorylated by Cdk1. Expression of S-phase and M/G1 transcripts are downregulated when phosphorylation of these factors is blocked, demonstrating that Cdk1 promotes expression of late cell cycle genes.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL16244

32 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL9377 GPL14887

56 Samples

Download data: PAIR

(Submitter supplied) The replication of eukaryotic chromosomes is organized temporally and spatially within the nucleus through epigenetic regulation of replication origin function. The characteristic initiation timing of specific origins is thought to reflect their chromatin environment or sub-nuclear positioning, however the mechanism remains obscure. Here we show that the yeast Forkhead transcription factors, Fkh1 and Fkh2, are global determinants of replication origin timing. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array; Other

Platform:

GPL14887

30 Samples

Download data: PAIR

(Submitter supplied) The replication of eukaryotic chromosomes is organized temporally and spatially within the nucleus through epigenetic regulation of replication origin function. The characteristic initiation timing of specific origins is thought to reflect their chromatin environment or sub-nuclear positioning, however the mechanism remains obscure. Here we show that the yeast Forkhead transcription factors, Fkh1 and Fkh2, are global determinants of replication origin timing. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9377

26 Samples

Download data: TXT

(Submitter supplied) Forkhead box (FOX) transcription factors regulate a wide variety of cellular functions in higher eukaryotes, including cell cycle control and developmental regulation. In Saccharomyces cerevisiae, Forkhead proteins Fkh1 and Fkh2 perform analogous functions, regulating genes involved in cell cycle control, while also regulating matingtype silencing and switching involved in gamete development. Recently, we revealed a novel role for Fkh1 and Fkh2 in the regulation of replication origin initiation timing, which, like donor preference in mating-type switching, appears to involve long-range chromosomal interactions, suggesting roles for Fkh1 and Fkh2 in chromatin architecture and organization. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL14887

30 Samples

Download data: PAIR

(Submitter supplied) Two homeodomain proteins, Yox1 and Yhp1, act as repressors at early cell cycle boxes (ECBs) to restrict their activity to the M/G1 phase of the cell cycle in budding yeast. These proteins bind to Mcm1 and to a typical homeodomain binding site. The expression of Yox1 is periodic and directly correlated with its binding to, and repression of, ECB activity. The absence of Yox1 and Yhp1 or the constitutive expression of Yox1 leads to the loss of cell-cycle regulation of ECB activity. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2318

Platform:

GPL1914

13 Samples

Download data: GPR

(Submitter supplied) Yeast cell cycle microarray to identify global trancription profile. Keywords: cell cycle time course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2347

Platform:

GPL1914

13 Samples

Download data: GPR

Analysis of wild type W303 cells across two cell cycles, a length of 2 hours after synchronization with alpha factor. Results compared to those from an experiment using a yox1 yhp1 double mutant strain (GDS2318).

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 13 time sets

Platform:

GPL1914

Series:

GSE3635

13 Samples

Download data: GPR

Analysis of yox1 yhp double mutants across two cell cycles, a length of 2 hours after synchronization with alpha factor. Results compared to those from an experiment using wild type cells (GDS2347) and provide insight into the role of Yox1 and Yhp1 in early cell cycle box-dependent transcription.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log10 ratio, 13 time sets

Platform:

GPL1914

Series:

GSE5283

13 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

81 Samples

Download data: CEL

(Submitter supplied) Genetic and environmental factors influence the phenotype of an organism. Time is rarely considered when studying changes in cellular phenotype. Time-resolved microarray data revealed genome-wide transcriptional changes in cells oscillating with ~2 and ~4 h periods. We mapped the global patterns of transcriptional oscillations into a 3-dimensional map to represent different cellular phenotypes of oscillation period. more...

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

49 Samples

Download data: CEL

(Submitter supplied) Genetic and environmental factors influence the phenotype of an organism. Time is rarely considered when studying changes in cellular phenotype. Time-resolved microarray data revealed genome-wide transcriptional changes in cells oscillating with ~2 and ~4 h periods. We mapped the global patterns of transcriptional oscillations into a 3-dimensional map to represent different cellular phenotypes of oscillation period. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

32 Samples

Download data: CEL