GEO DataSets

(Submitter supplied) By exploring the large-scale transcriptome analysis, we aim to identify differentially expressed genes in the comparison between resistant and susceptible families.

Organism:

Pinus sylvestris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32142

24 Samples

Download data: TXT

(Submitter supplied) The meristem-associated endosymbiont M. extorquens DSM13060 significantly increases needle and root growth of Scots pine (Pinus sylvestris L.) seedlings without producing plant hormones, but by aggregating around host nuclei. Here we studied gene expression of the pine host induced by M. extorquens DSM13060 infection. We selected the time point of 90 days post-inoculation for our analysis based, because at this point, Methylorubrum extorquens DSM13060 has systemically colonized the pine seedlings, being found throughout tissues of roots and shoots.

Organism:

Pinus sylvestris; Pinus pinaster

Type:

Expression profiling by array

Platform:

GPL29939

6 Samples

Download data: GPR, TXT, XLS, XLSX

(Submitter supplied) In conifer forests of Northern Europe, a pathogenic fungus Heterobasidion annosum attacks the roots of Scots pine and causes mortality. Trees with infection grow slower and produce less timber with reduced quality. Despite applied control methods, such as switching tree species to a non-host species, or stump treatment, root and butt rot continues to be a serious forest health problem. Disease resistance breeding is a less-applied control method which has potential to improve tree health. more...

Organism:

Pinus sylvestris

Type:

Expression profiling by array

Platform:

GPL19078

24 Samples

Download data: PAIR

(Submitter supplied) Illumina HiSeq technology was used to generate mRNA profiles from Cenococcum geophilum ectomycorrhizal Pine roots, sclerotia and extramatrical mycelium compared to free-living mycelium . Ectomycorrhizal pine roots, sclerotia, extramatrical mycelium and control mycelium were harvested after 90 days and used for RNA extraction. Reads of 150bp were generated and aligned to the C. geophilum reference genome (https://genome.jgi.doe.gov/Cenge3/Cenge3.home.html).

Organism:

Pinus sylvestris; Cenococcum geophilum 1.58

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24479 GPL24473 GPL24474

12 Samples

Download data: TXT

(Submitter supplied) Understanding of mechanisms of resistance of forest trees against microbial pathogens is an essential prerequisite for the development of sustainable forestry practices and for the improvement of commercially-grown trees via either conventional breeding or rational genetic engineering. We have studied the transcriptional response of Scots pine trees to Heterobasidion annosum infection under field conditions. more...

Organism:

Pinus sylvestris

Type:

Expression profiling by array

Platform:

GPL22554

6 Samples

Download data: PAIR

(Submitter supplied) To identify specific gene networks induced in host roots by C. geophilum, we inoculated seedlings of Scots pine simultaneously with C. geophilum and either Suillus granulatus or Rhizopogon roseolus, two common ECM fungi associated to pines. We then measured the differential expression of Scots pine genes in the respective mycorrhizas using oligoarrays.

Organism:

Pinus sylvestris; Cenococcum geophilum

Type:

Expression profiling by array

Platform:

GPL21757

14 Samples

Download data: PAIR

(Submitter supplied) Coniferous trees use the oleoresin, a mixture of non-volatile non-volatile diterpene acids and a large (20-50%) volatile fraction of mono- and sesquiterpenes, as a defence tool against damaging herbivores and pathogens. Volatilization of monoterpenes increases viscosity of oleoresin and finally leading to polymerization of resin and a formation of protective solid plug. Resin storing conifers form an important source of volatile organic compounds (VOCs) mainly dominated by volatile monoterpenes. more...

Organism:

Pinus sylvestris

Type:

Expression profiling by array

Platform:

GPL19078

6 Samples

Download data: PAIR

(Submitter supplied) The experiment was designed to enable validation of pre-processing and test algorithms. The in-house produced cDNA-array contains 44 clones: 19 clones from mice, 4 clones from Pine (involved in photosynthesis) and 21 artificial clones from the Lucidea Universal ScoreCard (Amersham Bioscienses), where each clone was printed 480 times in 48 identically designed sub-grids. The experiment contains eight arrays with identical experimental design. more...

Organism:

Pinus sylvestris; Mus musculus

Type:

Expression profiling by array

Platform:

GPL13623

8 Samples

Download data: CSV, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pinus taeda; Pinus sylvestris

Type:

Expression profiling by array

Platforms:

GPL1508 GPL4039

108 Samples

Download data: TXT

(Submitter supplied) The response mechanisms, recognition and specificity of conifer trees during interaction with pathogenic, saprotrophic or symbiotic ectomycorrhizal fungus were investigated. The roots of Pinus sylvestris were challenged for five days with either Heterobasidion annosum (a pathogenic root rot fungus which attacks Norway spruce, Scots pine and broad leaf trees); Laccaria bicolor (an obligate ectomycorrhizal symbiont); or Trichoderma aureoviride (an obligate saprotroph). more...

Organism:

Pinus taeda; Pinus sylvestris

Type:

Expression profiling by array

Platform:

GPL4039

36 Samples

Download data: TXT

(Submitter supplied) The response mechanisms, recognition and specificity of conifer trees during interaction with pathogenic, saprotrophic or symbiotic ectomycorrhizal fungus were investigated. The roots of Pinus sylvestris were challenged for fifteen days with either Heterobasidion annosum (a pathogenic root rot fungus which attacks Norway spruce, Scots pine and broad leaf trees); Laccaria bicolor (an obligate ectomycorrhizal symbiont); or Trichoderma aureoviride (an obligate saprotroph). more...

Organism:

Pinus taeda; Pinus sylvestris

Type:

Expression profiling by array

Platform:

GPL4039

36 Samples

Download data: TXT

(Submitter supplied) Comparative transcriptome analysis of early interaction events in Scots pine root tissues following challenge with a pathogenic, saprophytic or symbiotic fungus. Seedlings of P. sylvestris (19 days post germination) were transferred to wet, sterile filter paper on Petri-plates. Thereafter, the roots of the seedlings were inoculated with the mycelial homogenate of either Heterobasidion annosum (FP5, P-type) a pathogenic root rot fungus which attacks Norway spruce, Scots pine and broad leaf trees or Laccaria bicolor, an obligate ectomycorrhizal symbiont or Trichoderma aureoviride- an obligate saprotroph. more...

Organism:

Pinus sylvestris; Pinus taeda

Type:

Expression profiling by array

Platform:

GPL1508

36 Samples

Download data

(Submitter supplied) The probe sequences are available in the file: NimbleGen Pine 12K array v1.0.ndf. Protocol: Propriety in situ oligonucleotide synthesis

Organism:

Pinus sylvestris

1 Series

6 Samples

Download data: NDF, NGD

(Submitter supplied) This is a condensed platform of the full array which has probes designed from Cenococcum geophilum and Pinus sylvestris 454 sequences. Native array description file: 100616_CG-Pinus_EXP.ndf Design name: 100616_CG-Pinus_EXP Design ID: 532363 Protocol: Propriety in situ oligonucleotide synthesis.

Organism:

Pinus sylvestris; Cenococcum geophilum

1 Series

14 Samples

Download data: NDF

(Submitter supplied) NimbleGen Pine 36K array v1.0.ndf NimbleGen Pine 36K array v1.0.ngd designname=110224_Pinus_FA_exp designid=549411 Protocol: Propriety in situ oligonucleotide synthesis

Organism:

Pinus sylvestris

2 Series

30 Samples

Download data: NDF, NGD

(Submitter supplied) The in-house produced cDNA-array contains 44 clones: 19 clones from mice, 4 clones from Pine (involved in photosynthesis) and 21 artificial clones from the Lucidea Universal ScoreCard (Amersham Bioscienses), where each clone was printed 480 times in 48 identically designed sub-grids. Protocol: The clones were dissolved in 50% DMSO and printed using 2500K pins (Biorobotics) with a MicroGrid II 610 instrument (Biorobotics) on Ultra GAPS slides (Corning). more...

Organism:

Pinus sylvestris; Mus musculus

1 Series

8 Samples

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