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| Status |
Public on Dec 31, 2008 |
| Title |
Contribution of HSD17B12 for estradiol biosynthesis in human breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
17beta-hydroxysteroid dehydrogenase type12 (HSD17B12) has been demonstrated to be involved in regulation of in situ biosynthesis of estradiol (E2). HSD17B12 expression was reported in breast carcinomas but its functions have remained unknown. Therefore, we examined the correlation between mRNA expression profiles determined by microarray analysis and tissue E2 concentrations obtained from 16 postmenopausal breast carcinoma cases in order to analyze an association of the enzyme expression with intratumoral E2 production. No significant correlations were detected between intratumoral HSD17B12expression and E2 concentration.These findings suggest that the presence of HSD17B12 in carcinoma cells contributes to a development of human breast carcinoma via a pathway other than in situ E2 biosynthesis.
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| Overall design |
Gene expression profiles of LCM samples in isolated carcinoma cells in 16 breast carcinoma cases were examined using microarray analysis. Breast carcinomas were rapidly embedded in Tissue-Tek optimal cutting temperature compound (Sakura Finetechnical Co. Ltd., Tokyo, Japan) and frozen sectioned at a thickness of 8micro m. Approximately 5000 cells were laser transferred from these isolated specimens. Isolated total RNA was applied microarray analysis. Sample preparation and processing were performed essentially as described in the Affymetrix GeneChip Expression Analysis Manual (Affymetix, Inc., Santa Clara, CA). The labeled cRNA samples were hybridized to the complete human U133 GeneChip set (Affymetrix), including 22,215 and 22,577 genes. In our present study, we focused on mRNA expression of 156 genes which is annotated according to Gene Ontology database (steroid biosynthetic process, accession No.6694) as genes involved in steroid biosynthesis, and HSD17B12 unregistered in Gene Ontology accession No.6694.
The gene expression profiles of the patients with breast cancer in which tissue estradiol was under detection limit (2 patients) was averaged, and applied to an analysis. 15 gene expression profiles of 16 breast carcinomas examined were arranged according to the levels of tissue estradiol concentration, and the clustering analysis between each gene was performed using GeneSpring GX 7.3.1 (Agilent Technologies, Waldbronn, Germany).
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| Contributor(s) |
Nagasaki S, Suzuki T, Miki Y, Kitada K, Ishida T, Ohuchi N, Sasano H |
| Citation(s) |
19190350, 21988594 |
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| Submission date |
Jul 02, 2008 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Shuji Nagasaki |
| Organization name |
Tohoku University Graduate School of Medicine
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| Street address |
2-1 Seiryo-machi, Aoba-ku
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| City |
Sendai |
| State/province |
Miyagi-ken |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (32)
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| GSM302816 |
Human-LMD dissected breast cancer 1A |
| GSM302817 |
Human-LMD dissected breast cancer 1B |
| GSM302818 |
Human-LMD dissected breast cancer 2A |
| GSM302819 |
Human-LMD dissected breast cancer 2B |
| GSM302821 |
Human-LMD dissected breast cancer 3A |
| GSM302842 |
Human-LMD dissected breast cancer 3B |
| GSM302843 |
Human-LMD dissected breast cancer 4A |
| GSM302845 |
Human-LMD dissected breast cancer 4B |
| GSM302847 |
Human-LMD dissected breast cancer 5A |
| GSM302848 |
Human-LMD dissected breast cancer 5B |
| GSM302850 |
Human-LMD dissected breast cancer 6A |
| GSM302851 |
Human-LMD dissected breast cancer 6B |
| GSM302852 |
Human-LMD dissected breast cancer 7A |
| GSM302853 |
Human-LMD dissected breast cancer 7B |
| GSM302854 |
Human-LMD dissected breast cancer 8A |
| GSM302855 |
Human-LMD dissected breast cancer 8B |
| GSM302856 |
Human-LMD dissected breast cancer 9A |
| GSM302857 |
Human-LMD dissected breast cancer 9B |
| GSM302858 |
Human-LMD dissected breast cancer 10A |
| GSM302860 |
Human-LMD dissected breast cancer 10B |
| GSM302861 |
Human-LMD dissected breast cancer 11A |
| GSM302862 |
Human-LMD dissected breast cancer 11B |
| GSM302863 |
Human-LMD dissected breast cancer 12A |
| GSM302865 |
Human-LMD dissected breast cancer 12B |
| GSM302867 |
Human-LMD dissected breast cancer 13A |
| GSM302868 |
Human-LMD dissected breast cancer 13B |
| GSM302869 |
Human-LMD dissected breast cancer 14A |
| GSM302871 |
Human-LMD dissected breast cancer 14B |
| GSM302873 |
Human-LMD dissected breast cancer 15A |
| GSM302875 |
Human-LMD dissected breast cancer 15B |
| GSM302877 |
Human-LMD dissected breast cancer 16A |
| GSM302879 |
Human-LMD dissected breast cancer 16B |
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| Relations |
| BioProject |
PRJNA105835 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11965_RAW.tar |
298.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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