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| Status |
Public on May 10, 2021 |
| Title |
Microarray and qPCR Analysis of Mitochondrial Metabolism Activation during Prenatal and Early Postnatal Development in Rats and Humans with Emphasis on CoQ10 Biosynthesis |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
During the mammalian ontogenesis, a rapid switch from glycolytic metabolism to oxidative phosphorylation must proceed during postnatal adaptation to extra-uterine conditions. It was proposed that in the course of intra-uterine fetal development, expression changes proceed to cover all metabolic demands all along this critical and extremely fast period. Using microarray techniques, quantitative PCR, enzyme activities’ and coenzyme Q content measurements, we describe perinatal mitochondrial metabolism acceleration in rat liver and skeletal muscle between the 16th day of gestation and the first days of life and possible correlation with results in human. Out of 1546 mitochondrial genes, 72% and 53% were significantly changed in liver and skeletal muscle, respectively. The most important expression shift occurred in the liver at least two days before parturition.
We used microarrays to detail the orchestration of gene expression underlying perinatal metabolic shift and identified up- and down-regulated genes during this process.
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| Overall design |
Rattus norvegicus perinatal liver and skeletal muscle tissues were selected as a model of mammalian adaptation to extra-uterine conditions. To study gene expression, we used Affymetrix microarrays. Analysis wasperformed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada. For analysis we used tissues at five time-points: fetal day 16 (F16), fetal day 20 (F20), last day before parturition - fetal day 22 (F22), first postnatal day (P1) and 4th postnatal day (P4). We obtained also data from fetal day 18 (in skeletal muscle) and 90th day of life (liver, “adult” control), which were not used in analysis, but are part of this data submission. For liver tissue analysis, we used four biological replicates per time-point. For skeletal muscle analysis, five biological replicates were used.
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| Web link |
https://doi.org/10.3390/biology10050418
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| Contributor(s) |
Krizova J, Hulkova M, Capek V, Mlejnek P, Silhavy J, Zeman J, Hansikova H |
| Citation(s) |
34066731 |
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| Submission date |
May 10, 2019 |
| Last update date |
Nov 04, 2022 |
| Contact name |
Jana Krizova |
| E-mail(s) |
[email protected]
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| Organization name |
Charles University in Prague, First Faculty of Medicine, and General University Hospital in Prague
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| Department |
Department of Pediatrics and Inherited Metabolic Disorders
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| Lab |
Laboratory for Study of Mitochondrial Disorders
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| Street address |
Ke Karlovu 2
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| City |
Prague 2 |
| State/province |
Non United States Or Canada |
| ZIP/Postal code |
12808 |
| Country |
Czech Republic |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (54)
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| GSM3759857 |
fetal liver at F16, biological rep4 |
| GSM3759858 |
fetal liver at F20, biological rep1 |
| GSM3759859 |
fetal liver at F20, biological rep2 |
| GSM3759860 |
fetal liver at F20, biological rep3 |
| GSM3759861 |
fetal liver at F20, biological rep4 |
| GSM3759862 |
fetal liver at F22, biological rep1 |
| GSM3759863 |
fetal liver at F22, biological rep2 |
| GSM3759864 |
fetal liver at F22, biological rep3 |
| GSM3759865 |
fetal liver at F22, biological rep4 |
| GSM3759866 |
postnatal liver at P1, biological rep1 |
| GSM3759867 |
postnatal liver at P1, biological rep2 |
| GSM3759868 |
postnatal liver at P1, biological rep3 |
| GSM3759869 |
postnatal liver at P1, biological rep4 |
| GSM3759870 |
postnatal liver at P4, biological rep1 |
| GSM3759871 |
postnatal liver at P4, biological rep2 |
| GSM3759872 |
postnatal liver at P4, biological rep3 |
| GSM3759873 |
postnatal liver at P4, biological rep4 |
| GSM3759874 |
postnatal adult liver, biological rep1 |
| GSM3759875 |
postnatal adult liver, biological rep2 |
| GSM3759876 |
postnatal adult liver, biological rep3 |
| GSM3759877 |
postnatal adult liver, biological rep4 |
| GSM3759878 |
fetal skeletal muscle at F16, biological rep1 |
| GSM3759879 |
fetal skeletal muscle at F16, biological rep2 |
| GSM3759880 |
fetal skeletal muscle at F16, biological rep3 |
| GSM3759881 |
fetal skeletal muscle at F16, biological rep4 |
| GSM3759882 |
fetal skeletal muscle at F16, biological rep5 |
| GSM3759883 |
fetal skeletal muscle at F20, biological rep1 |
| GSM3759884 |
fetal skeletal muscle at F20, biological rep2 |
| GSM3759885 |
fetal skeletal muscle at F20, biological rep3 |
| GSM3759886 |
fetal skeletal muscle at F20, biological rep4 |
| GSM3759887 |
fetal skeletal muscle at F20, biological rep5 |
| GSM3759888 |
fetal skeletal muscle at F22, biological rep1 |
| GSM3759889 |
fetal skeletal muscle at F22, biological rep2 |
| GSM3759890 |
fetal skeletal muscle at F22, biological rep3 |
| GSM3759891 |
fetal skeletal muscle at F22, biological rep4 |
| GSM3759892 |
fetal skeletal muscle at F22, biological rep5 |
| GSM3759893 |
postnatal skeletal muscle at P1, biological rep1 |
| GSM3759894 |
postnatal skeletal muscle at P1, biological rep2 |
| GSM3759895 |
postnatal skeletal muscle at P1, biological rep3 |
| GSM3759896 |
postnatal skeletal muscle at P1, biological rep4 |
| GSM3759897 |
postnatal skeletal muscle at P1, biological rep5 |
| GSM3759898 |
postnatal skeletal muscle at P4, biological rep1 |
| GSM3759899 |
postnatal skeletal muscle at P4, biological rep2 |
| GSM3759900 |
postnatal skeletal muscle at P4, biological rep3 |
| GSM3759901 |
postnatal skeletal muscle at P4, biological rep4 |
| GSM3759902 |
postnatal skeletal muscle at P4, biological rep5 |
| GSM3759903 |
fetal skeletal muscle at F18, biological rep1 |
| GSM3759904 |
fetal skeletal muscle at F18, biological rep2 |
| GSM3759905 |
fetal skeletal muscle at F18, biological rep3 |
| GSM3759906 |
fetal skeletal muscle at F18, biological rep4 |
| GSM3759907 |
fetal skeletal muscle at F18, biological rep5 |
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| Relations |
| BioProject |
PRJNA542327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131012_RAW.tar |
232.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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