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| Status |
Public on Mar 01, 2009 |
| Title |
DCC binding and function (ChIP-chip: DPY-27) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
In many species, a dosage compensation complex (DCC) is targeted to X chromosomes of one sex to equalize levels of X gene products between males (1X) and females (2X). Here we identify cis-acting regulatory elements that target the C. elegans X chromosome for repression by the DCC. The DCC binds to discrete, dispersed sites on X of two types. rex sites recruit the DCC in an autonomous, DNA sequence-dependent manner using a 12 bp consensus motif that is enriched on X. This motif is critical for DCC binding, is clustered in rex sites, and confers much of X-chromosome specificity. Motif variants enriched on X by 3.8-fold or more are highly predictive (95%) for rex sites. In contrast, dox sites lack the X-enriched variants and cannot bind the DCC when detached from X. dox sites are more prevalent than rex sites and, unlike rex sites, reside preferentially in promoters of some expressed genes. These findings fulfill predictions for a targeting model in which the DCC binds to recruitment sites on X and disperses to discrete sites lacking autonomous recruitment ability. To relate DCC binding to function, we identified dosage-compensated and non-compensated genes on X. Unexpectedly, many genes of both types have bound DCC, but many do not, suggesting the DCC acts over long distances to repress X gene expression. Remarkably, the DCC binds to autosomes, but at far fewer sites and rarely at consensus motifs. DCC disruption causes opposite effects on expression of X and autosomal genes. The DCC thus acts at a distance to impact expression throughout the genome.
Keywords: dosage compensation, condensin, X chromosome, gene expression, epigenetics, C. elegans
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| Overall design |
ChIP-chip with DPY-27
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| Contributor(s) |
Jans J, Gladden JM, Ralston EJ, Pickle CS, Michel AH, Pferdehirt RR, Eisen MB, Meyer BJ |
| Citation(s) |
19270160 |
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| Submission date |
Jan 30, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Barbara J. Meyer |
| E-mail(s) |
[email protected]
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| Phone |
510 643 5583
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| Organization name |
HHMI/UCB
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| Department |
MCB
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| Lab |
Meyer
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| Street address |
16 Barker Hall #3204
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL7098 |
NimbleGen C_elegans_WS170_Tiling_Iso_HX1 tiling design |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE14640 |
A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome |
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| Relations |
| BioProject |
PRJNA114415 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14651_RAW.tar |
229.7 Mb |
(http)(custom) |
TAR (of GFF, PAIR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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