|
| Status |
Public on Mar 01, 2009 |
| Title |
N2_DPY-27_HD-IP1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
N2 embryo input
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
N2 XX embryo
nonselected input
|
| Treatment protocol |
Embryos used for microarray analysis were obtained from bleached gravid adults.
|
| Growth protocol |
Wild type, N2, Caenorhabditis elegans were grown on NG pates suplimented with 100 µg/ml carbinicillin and 1 µg/ml IPTG seeded with E. coli HT115 (DE3)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
see PDF linked to GSE14640
|
| Label |
Cy3
|
| Label protocol |
performed at Nimblegen using their standard protocol (http://www.nimblegen.com/products/chip/index.html).
|
| |
|
| Channel 2 |
| Source name |
N2 embryo DPY-27 IP
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
N2 XX embryo
DPY27 IP
|
| Treatment protocol |
Embryos used for microarray analysis were obtained from bleached gravid adults.
|
| Growth protocol |
Wild type, N2, Caenorhabditis elegans were grown on NG pates suplimented with 100 µg/ml carbinicillin and 1 µg/ml IPTG seeded with E. coli HT115 (DE3)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
see PDF linked to GSE14640
|
| Label |
Cy5
|
| Label protocol |
performed at Nimblegen using their standard protocol (http://www.nimblegen.com/products/chip/index.html).
|
| |
|
| |
| Hybridization protocol |
performed at Nimblegen using their standard protocol (http://www.nimblegen.com/products/chip/index.html).
|
| Scan protocol |
performed at Nimblegen using their standard protocol (http://www.nimblegen.com/products/chip/index.html).
|
| Description |
N/A
|
| Data processing |
performed at Nimblegen using their standard protocol producing gff files with log2 ratios of experimental/input (http://www.nimblegen.com/products/chip/index.html).
|
| |
|
| Submission date |
Jan 30, 2009 |
| Last update date |
Feb 02, 2009 |
| Contact name |
Barbara J. Meyer |
| E-mail(s) |
[email protected]
|
| Phone |
510 643 5583
|
| Organization name |
HHMI/UCB
|
| Department |
MCB
|
| Lab |
Meyer
|
| Street address |
16 Barker Hall #3204
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL7098 |
| Series (2) |
| GSE14640 |
A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome |
| GSE14651 |
DCC binding and function (ChIP-chip: DPY-27) |
|
