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| Status |
Public on Apr 14, 2009 |
| Title |
MEF wt versus Med23-/- KO after 20% Serum Stimulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Affymetrix MOE430A arrays. Mouse embryonic fibroblasts (MEFs) from E11 passaged through crisis. Starved in 0.5% FBS for 16 h then stimulated by 20% serum final concentration for 30 minutes. The purpose was to identify genes affected by the loss of MED23 protein (subunit of the mediator complex). Samples were assayed in duplicate (Set 1 and Set 2) for starved state and serum state cells.
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| Overall design |
Cells starved in low-serum (0.5%) for 16 h then add final conc. 20% FBS for 30 minutes. Harvest total RNA from duplicate samples. One sample set is kept starved and serves as the baseline. The other set is stimulated. This is done for both wild-type cell and the Med23-/- KO cells. We looked for gene expression fold changes greater than 2 (plus or minus) to find genes affected for serum stimulation by the loss of MED23, looking in detail at immediate early genes like Egr1.
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| Contributor(s) |
Stevens JL, Balamotis MA, Berk AJ |
| Citation(s) |
19417216 |
| |
| Submission date |
Apr 10, 2009 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Michael Andrew Balamotis |
| E-mail(s) |
[email protected]
|
| Organization name |
Lawrence Berkeley National Lab
|
| Department |
Life Sciences
|
| Lab |
Kohwi-Shigematsu
|
| Street address |
1 Cycotron Rd. Bld. 84
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (8)
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| GSM391369 |
MEF wt cells serum starved 16 h Set 1 |
| GSM391370 |
MEF wt cells serum starved 16 h Set 2 |
| GSM391371 |
MEF Med23-/- (KO) cells serum starved 16 h Set 1 |
| GSM391372 |
MEF Med23-/- (KO) cells serum starved 16 h Set 2 |
| GSM391373 |
MEF wt cells 20% serum stimulated Set 1 |
| GSM391374 |
MEF wt cells 20% serum stimulated Set 2 |
| GSM391379 |
MEF Med23-/- (KO) cells 20% serum stimulated Set 1 |
| GSM391382 |
MEF Med23-/- (KO) cells 20% serum stimulated Set 2 |
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| Relations |
| BioProject |
PRJNA115639 |
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