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| Status |
Public on Sep 02, 2021 |
| Title |
Evaluation of changes in microRNA expression in naïve and influenza-virus specific CD8+ T cells |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNA microarray expression dataset evaluating relative changes in microRNA expression levels between naïve and effector OT-I CD8+ T cells during influenza virus infection in mice.
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| Overall design |
Naïve OT-I CD8+ T cells were FACS sorted (based on CD44- and CD62L+ expression) from the spleens on uninfected female OT-I mice. Naïve cells were kept for analysis of microRNA expression in naïve OT-I CD8+ T cells. Additionally, 1x10e4 cells were adoptively transferred into uninfected female C57BL/6 mice (via tail vein injection) followed by intranasal infection with WSN-OVA influenza virus. At day 9, donor OT-I cells were FACS sorted from the lungs of influenza virus-infected mice for analysis of microRNA expression in effector OT-I CD8+ T cells. Total RNA was isolated from sorted cells and miRNA gene expression was assessed using an Affymetrix murine miRNA 4.0 gene chip. CEL files were normalized and analyzed using Affymetrix Expression Console, and miRNA annotation is from miRBASE version 21.
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| Contributor(s) |
Katsikis PD, Hope JL |
| Citation(s) |
35022277 |
| |
| Submission date |
Aug 31, 2021 |
| Last update date |
May 12, 2023 |
| Contact name |
Jennifer Hope |
| E-mail(s) |
[email protected]
|
| Organization name |
Sanford Burnham Prebys Medical Discovery Institute
|
| Street address |
10901 N Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92126 |
| Country |
USA |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA759265 |
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