|
| Status |
Public on Dec 23, 2009 |
| Title |
Expression profile of early stage druing induced pluripotency regulated by BMP/FBS |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
FBS and BMP influence the somatic reprogramming induced by Oct4/Sox2/Klf4/c-Myc similarly. Bone morphogenetic proteins (BMPs) are abundant in serum and activate Smad1/5/8 to regulated target genes.
BMPs play a important role in ES self-renewal, neurogenesis, osteogenesis, angiogenesis. We performed this experiment for identify what targets are regulated by BMPs and FBS.
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| Overall design |
We performed a expression profile analysis at D3 after reprogramming factor transduction in various media. The media include standard medium for ES culture (mES), serum free medium for iPS (iPS-SF1), iPS-SF1 supplemented with BMP4, iPS-SF1 supplemented with FBS, iPS-SF1 supplemented with BMP4 and Noggin. Three biological repeats were perfromed.
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| |
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| Contributor(s) |
Chen J, Pei D, He W |
| Citation(s) |
23202127 |
| |
| Submission date |
Dec 22, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Duanqing Pei |
| E-mail(s) |
[email protected]
|
| Phone |
086-20-32290520
|
| Fax |
086-20-32290606
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| Organization name |
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
|
| Department |
Center for Molecular Medicine
|
| Lab |
Lingwen Zeng's Lab
|
| Street address |
Room 301, International Business Incubator, Guangzhou science park
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510663 |
| Country |
China |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (15)
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| Relations |
| BioProject |
PRJNA122439 |
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