|
| Status |
Public on Dec 23, 2009 |
| Title |
BMP_Nog-1 |
| Sample type |
RNA |
| |
|
| Source name |
iPS indcued by OKSM cultured in iPS-SF1 supplemented with BMP4 and Noggin at D3 post-infection
|
| Organism |
Mus musculus |
| Characteristics |
cell line: mouse embryonic fibroblast cells infected by Oct4/Sox2/Klf4/c-Myc
|
| Treatment protocol |
MEFs were infected with Oct4/Sox2/Klf4/c-Myc retrovirus and then cultured in indicated media.
|
| Growth protocol |
Cultured in 37℃ with 5% CO2 in the media descripted in sample source name.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Whole Transcript (WT) Sense Target Labeling Assay Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip MoGene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
expression data from iPS cells at D3 post-retroviral infection
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Partek software.
|
| |
|
| Submission date |
Dec 22, 2009 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Duanqing Pei |
| E-mail(s) |
[email protected]
|
| Phone |
086-20-32290520
|
| Fax |
086-20-32290606
|
| Organization name |
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
|
| Department |
Center for Molecular Medicine
|
| Lab |
Lingwen Zeng's Lab
|
| Street address |
Room 301, International Business Incubator, Guangzhou science park
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510663 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE19604 |
Expression profile of early stage druing induced pluripotency regulated by BMP/FBS |
|
