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| Status |
Public on Jun 12, 2010 |
| Title |
Gene expression in mouse neonatal cardiomyocytes, cardiac fibroblasts, reprogramming failed GFP- cells, and GFP+ iCMs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The reprogramming of fibroblast cells to induced pluripotent stem (iPS) cells raises the possibility that a somatic cell could be reprogrammed to an alternative differentiated fate without first becoming a stem/progenitor cell. A large pool of fibroblast cells exists in the post-natal heart, yet no single “master regulator” of direct cardiac reprogramming has been identified. Here, we report that a combination of three developmental transcription factors (i.e., Gata4, Mef2c and Tbx5) rapidly and efficiently reprogrammed post-natal cardiac or tail-tip fibroblasts directly into differentiated cardiomyocyte-like cells. Induced cardiomyocytes expressed cardiac-specific markers, had a global gene expression profile similar to cardiomyocytes, and contracted spontaneously. Fibroblast cells transplanted into mouse hearts one day after transduction of the three factors also differentiated into cardiomyocyte-like cells. These findings demonstrate that functional cardiomyocytes can be directly reprogrammed from differentiated somatic cells by defined factors. Reprogramming of endogenous or explanted fibroblast cells might provide a source of cardiomyocytes for regenerative approaches.
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| Overall design |
To compare the progressive global gene expression pattern of iCMs, cardiomyocytes and cardiac fibroblasts, we isolated RNA from mouse neonatal cardiomyocytes, cardiac fibroblasts, reprogramming failed GFP- cells and GFP+ iCMs, and profiled mRNA expressions by microarray analyses. Arrays were performed using Affymetrix mouse Gene 1.0 ST arrays. Analysis was performed on three biological replicates for each group.
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| Contributor(s) |
IEDA M, SRIVASTAVA D |
| Citation(s) |
20691899 |
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| Submission date |
Jun 10, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Masaki Ieda |
| E-mail(s) |
[email protected]
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| Phone |
415-734-2880
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| Organization name |
Gladstone Institute
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| Street address |
1650 Owens street,
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94404 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| GSM554203 |
2 week reprogramming failed GFP- cells-rep1 |
| GSM554555 |
2 week reprogramming failed GFP- cells-rep2 |
| GSM554556 |
2 week reprogramming failed GFP- cells-rep3 |
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| Relations |
| BioProject |
PRJNA127407 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22292_RAW.tar |
77.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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