|
| Status |
Public on Jul 05, 2012 |
| Title |
Time Series of gene expression during the course of myogenic differentiation in mouse skeletal muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In skeletal myogenesis, the transcription factor MyoD activates distinct transcriptional programs in progenitors compared to terminally differentiated cells. Using ChIP-seq and gene expression analyses, we show that in primary myoblasts, Snail-HDAC1/2 repressive complex bind and exclude MyoD from its targets. Notably, Snail binds E-box motifs that are G/C-rich in their central dinucleotides, and such sites are almost exclusively associated with genes expressed during differentiation. By contrast, Snail does not bind the A/T-rich E-boxes associated with MyoD targets in myoblasts. Thus, Snai1-HDAC1/2 prevents MyoD occupancy on differentiation-specific regulatory elements and the change from Snail- to MyoD-binding often results in enhancer switching during differentiation. Furthermore, we show that a regulatory network involving Myogenic Regulatory Factors (MRFs), Snail/2, miR-30a and miR-206 acts as a molecular switch that controls entry into myogenic differentiation. Together, these results reveal a regulatory paradigm that directs distinct gene expression programs in progenitors versus terminally differentiated cells.
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| Overall design |
This data set contains 3 replicates of a myotube growth time series (0h, 2d, 5d). The data was RMA normalized per replicate set.
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| Contributor(s) |
Soleimani VD, Rudnicki MA |
| Citation(s) |
22771117, 30016497 |
| |
| Submission date |
Oct 19, 2010 |
| Last update date |
Jul 24, 2019 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (9)
|
| GSM610851 |
Myotubes after 2 days in differentiating medium - Replicate 1 |
| GSM610852 |
Myotubes after 2 days in differentiating medium - Replicate 2 |
| GSM610853 |
Myotubes after 2 days in differentiating medium - Replicate 3 |
| GSM610854 |
Myotubes after 5 days in differentiating medium - Replicate 1 |
| GSM610855 |
Myotubes after 5 days in differentiating medium - Replicate 2 |
| GSM610856 |
Myotubes after 5 days in differentiating medium - Replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE24904 |
Snail regulates MyoD binding-site occupancy to direct enhancer switching and differentiation-specific transcription in myogenesis |
|
| Relations |
| BioProject |
PRJNA133641 |
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