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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 09, 2011 |
| Title |
Cerebellum from mice exposed to chronic low-level chlorpyrifos oxon |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Chlorpyrifos (CPF) is an organophosphorus (OP) insecticide that is still widely used despite statutory restrictions on home use. CPF is converted to chlorpyrifos oxon (CPO) by oxidative desulfuration in liver. Paraoxonase (PON1) polymorphisms affects the catalytic efficiency of the hydrolysis of OPs, including CPO. We used both wt (PON1+/+) and PON1 knockout (PON1-/-) mice and PON1-/- mice carrying transgenes encoding the human alloforms tgHuPON1Q192 and tgHuPON1R192 to gain insight into the mechanisms of neurotoxicity of CPO throughout postnatal development, and to ascertain the importance of the PON1Q192R polymorphism for protecting against developmental toxicity of CPO. Whole-genome microarrays were used to measure gene expression changes associated with chronic CPO exposure of developing (PND 4-21) PON1-/-, tgHuPON1Q192R transgenic and PON1+/+ mice.
Expression profiles are derived from cerebella from wild-type C57/Bl6 and PON1-/- on a C57/Bl6 background and two transgenic strains (tgHuPON1Q192, tgHuPON1R192) expressing either human PON1Q192 or human PON1R192 on the PON1-/- C57/Bl6 background. The mice were subjected to chronic postnatal exposure to CPO (CPO).
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| Overall design |
Transgenic, PON1-KO and WT neonatal mice either treated with control (DMSO), 0.35 mg*kg-1*day-1 CPO or 0.5 mg*kg-1*day-1 CPO daily from PND 4 to PND 21. Chlorpyrifos (CPF) is converted to chlorpyrifos oxon (CPO) by oxidative desulfuration in liver.
55 arrays, 12 experimental groups (strain + treatment), due to QC issues the replicates are as follows; PON1-KO-0.35 (5), PON1-KO-.O5 (3), PON1-KO-DMSO (4), PON1-Q129-0.35 (5), PON1-Q129-0.5 (5), PON1-Q129-DMSO (4), PON1-R129-0.35 (5), PON1-R129-0.5 (5), PON1-R129-DMSO (4), WT-0.35 (6), WT-0.5 (3), WT-DMSO (6)
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| Contributor(s) |
Cole TB, Beyer RP, Bammler TK, Park SS, Farin FM, Costa LG, Furlong CE |
| Citation(s) |
21673326 |
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| Submission date |
Nov 09, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Michael Coon |
| E-mail(s) |
[email protected]
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| Phone |
(206)685-8774
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| Organization name |
University of Washington
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| Department |
Center for Ecogenetics and Environmental Health
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| Street address |
4225 Roosevelt Way N.E., Suite 100
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (55)
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| Relations |
| BioProject |
PRJNA134671 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25250_RAW.tar |
234.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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