|
| Status |
Public on Nov 09, 2011 |
| Title |
PON1.KO.0.35_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PON1.KO.0.35
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: Pon1-/-
tissue: cerebellum
age (at weaning): PND 22
|
| Biomaterial provider |
The University of Washington (Seattle, WA).
|
| Treatment protocol |
Chlorpyrifos (CPO) or vehicle (DMSO) injected S.Q.(1 uL/g bodyweight) daily from PND 4 to PND 21. CPO at 0.35 mg*kg-1*day-1 or 0.5 mg*kg-1*day-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bisected cerebellum were incubated in RNAlater for 12 h at 4oC, then stored at -20oC. Total RNA was isolated using TRIZOL Reagent and the QIAamp Tissue Kit from QIAGEN Inc., according to the manufacturer’s established protocols.
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix Mouse Genome ST 1.0 microarrays. Processing of the RNA samples was carried out using the Affymetrix GeneChip Whole Transcript Sense Target labeling protocol (for details see http://www.affymetrix.com/index.affx). The work was done in a core facility in the Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health at the University of Washington.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
|
| Description |
Toby_Cole_2267.3_13March09.CEL
|
| Data processing |
Affymetrix Expression Console v1.1 RMA normalization log2
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Nov 09, 2011 |
| Contact name |
Michael Coon |
| E-mail(s) |
[email protected]
|
| Phone |
(206)685-8774
|
| Organization name |
University of Washington
|
| Department |
Center for Ecogenetics and Environmental Health
|
| Street address |
4225 Roosevelt Way N.E., Suite 100
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25250 |
Cerebellum from mice exposed to chronic low-level chlorpyrifos oxon |
|
