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| Status |
Public on Jun 01, 2011 |
| Title |
Gene expression profiles of MCF7 with CARM1 knocked down |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this study is to identify ERalpha-target genes affected by knocking down of the histone arginine methyltransferase CARM1 in MCF7 breast cancer cells. The roles of CARM1 in ERalpha+ breast cancer was not well characterized. Therefore, we created a Dox inducible CARM1 knockingdown MCF7 cell line where CARM1 is decreased to 20% of endogeneous level to determine the
created a Dox-inducible CARM1shRNA overexpressing MCF7 cells for evaluation of the global effects of CARM1 on ERalpha-target gene expression.
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| |
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| Overall design |
MCF7-tet-on-CARM1shRNA clone 1 were treated under 4 conditions: DMSO; Dox; E2 (10nM); Dox+E2. In Dox+E2 condition, cells were pre-treated with Dox for 5 days before treating with E2 for 4 hours. 3 biological replicates were included and total of 12 samples were analyzed.
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| |
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| Contributor(s) |
Zhu Y, Wu J, Hua S, White K |
| Citation(s) |
21282336 |
| |
| Submission date |
Dec 21, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Wei Xu |
| Organization name |
UW-Madison
|
| Street address |
1400 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA135097 |
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