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| Status |
Public on Nov 06, 2024 |
| Title |
Expression data from human trisomy 21 and euploid fibroblasts, iPSCs, and neural progenitor cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Trisomy of human chromosome 21 (T21) gives rise to Down syndrome, the most frequent life-born autosomal aneuploidy. To enable in vitro analyses of the cellular and moelcular mechanisms leading to the neurological alterations associated with T21, we created and characterized a panel of genomically diverse T21 and euploid induced pluripotent stem cells (iPSCs) from fibroblasts obtained from the Coriell Institute for Biomedical Research, and we then differentiated these iPSCs into neural progenitor cells (NPCs).
Microarray transcriptomic analyses were performed on this panel of fibroblasts, iPSCs, and NPCs, identifying genes and pathways that were altered in T21 lines relative to euploid as well as genes and pathways in NPCs that showed inter-individual variability.
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| Overall design |
This study used cell lines obtained from racially diverse individuals with trisomy for human chromosome 21 along with age-and sex-matched euploid control cell lines. One RNA sample was collected per line. Fibroblast, iPSC, and NPC cDNA samples were hybridized to Affymetrix Clariom S HT arrays (902970, Thermofisher Scientific).
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| Contributor(s) |
Baxter L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 02, 2024 |
| Last update date |
Nov 07, 2024 |
| Contact name |
Laura L Baxter |
| Organization name |
NIH
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| Department |
NHGRI
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| Lab |
PGTS/CPHR
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| Street address |
35A Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL24324 |
[Clariom_S_Human_HT] Affymetrix Clariom S Assay HT, Human (Includes Pico Assay) |
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| Samples (51)
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| Relations |
| BioProject |
PRJNA1143483 |
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