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Status |
Public on Sep 30, 2011 |
Title |
Changes in the Bone Marrow Microenvironment Impact Anabolic Actions of PTH |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Irradiation induced bone marrow ablation ultimately enhanced PTH anabolic effects in bone. B6 mice at 10 days of age were sub-lethally irradiated and treated with PTH 24h later for 5 days. 24h post last-injection, bone marrow was flushed with Trizol and RNA isolated and purified. Microarray analyses was performed to determine differential differences in PTH effects in non-irradiated vs. irradiated bone marrow.
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Overall design |
Triplicates of 4 groups (total of 12 samples) which include: Nonirradiated Vehicle, Nonirradiated PTH, Irradiated Vehicle and Irradiated PTH
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Contributor(s) |
McCauley LK, Koh AJ |
Citation(s) |
22045660 |
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Submission date |
Apr 24, 2011 |
Last update date |
Mar 04, 2019 |
Contact name |
Amy J Koh |
Organization name |
University of Michigan
|
Department |
POM
|
Lab |
Laurie K. McCauley
|
Street address |
1011 N. University Ave.
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
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Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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Samples (12)
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GSM713688 |
whole bone marrow-nonirradiated-vehicle-rep1 |
GSM713689 |
whole bone marrow-nonirradiated-vehicle-rep2 |
GSM713690 |
whole bone marrow-nonirradiated-vehicle-rep3 |
GSM713691 |
whole bone marrow-nonirradiated-PTH-rep1 |
GSM713692 |
whole bone marrow-nonirradiated-PTH-rep2 |
GSM713693 |
whole bone marrow-nonirradiated-PTH-rep3 |
GSM713694 |
whole bone marrow-irradiated-vehicle-rep1 |
GSM713695 |
whole bone marrow-irradiated-vehicle-rep2 |
GSM713696 |
whole bone marrow-irradiated-vehicle-rep3 |
GSM713697 |
whole bone marrow-irradiated-PTH-rep1 |
GSM713698 |
whole bone marrow-irradiated-PTH-rep2 |
GSM713699 |
whole bone marrow-irradiated-PTH-rep3 |
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Relations |
BioProject |
PRJNA138773 |