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| Status |
Public on Jul 22, 2011 |
| Title |
Follicular B (FoB) cells purified from miR-17-92 transgenic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have generated a miR-17-92 transgenic allele (termed miR-17-92 Tg) whose expression can be turned on conditionally by Cre recombinase (Xiao et al, Nature Immunology, 9:405-14, 2008). The mice were crossed to CD19-Cre mice to turn on the transgene expression specifically in B cells.
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| Overall design |
Follicular B(FoB) cells were purified from CD19-Cre;miR-17~92 Tg/Tg mice (FoBtg1, FoBtg2, FoBtg3) and miR-17~92 Tg/Tg mice (FoBwt1, FoBwt2, FoBwt3) by MACS depletion of cells positive for CD5, CD43, and CD93 (also known as AA4.1).
The purity of follicular B cells was examined by flow cytometry and was greater than 95% for all samples. Total RNA was extracted using RNeasy kit (QIAGEN).
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| Contributor(s) |
Xiao C, Oda H, Head SR |
| Citation missing |
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| Submission date |
Jul 22, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Steven Head |
| E-mail(s) |
[email protected]
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| Phone |
858 784-2263
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| Organization name |
The Scripps Research Institute
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| Street address |
10550 N Torrey Pines Rd
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE56379 |
Follicular B (FoB) cells purified from miR-17~92 TG and miR-17~92 tKO mice |
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| Relations |
| BioProject |
PRJNA144263 |
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