|
| Status |
Public on Jul 22, 2011 |
| Title |
trans gene B(FoB) Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Follicular B(FoB) cells were purified from CD19-Cre;miR-17~92 Tg/Tg mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Follicular B (FoB)
genotype/variation: CD19-Cre;miR-17~92 Tg/Tg mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen Rneasy kits according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was generated using the NuGEN Applause WT Amp ST System. cDNA was fragmented and labeled using the NuGEN Encore Biotin Module.
|
| |
|
| Hybridization protocol |
Following fragmentation and end labeling, 5.0 ug of cDNA was hybridized for 16 hr at 45C on the Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G scanner.
|
| Description |
miR-17-92 transgenic allele (termed miR-17-92 Tg) whose expression can be turned on conditionally by Cre recombinase (Xiao et al, Nature Immunology, 9:405-14, 2008)
|
| Data processing |
The data were analyzed with RMA Express using the default settings (Background Adjust - Yes, Normalization - Quantile, Summarization Method - Median Polish, Store Residuals - unchecked). CDFRME file used for analysis: MoGene_CDFRME.zip.
|
| |
|
| Submission date |
Jul 22, 2011 |
| Last update date |
Jul 22, 2011 |
| Contact name |
Steven Head |
| E-mail(s) |
[email protected]
|
| Phone |
858 784-2263
|
| Organization name |
The Scripps Research Institute
|
| Street address |
10550 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE30877 |
Follicular B (FoB) cells purified from miR-17-92 transgenic mice |
| GSE56379 |
Follicular B (FoB) cells purified from miR-17~92 TG and miR-17~92 tKO mice |
|
