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| Status |
Public on Apr 10, 2006 |
| Title |
Gene expression during early Arabidopsis flower development |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment describes gene expression during early Arabidopsis flower development. We used a 35S:AP1-GR ap1 cal line to induce synchronized flower development by specifically activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as at one-day intervals for the following five days. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome microarrays (Operon).
Keywords: time course
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| Overall design |
Four sets of biologically independent tissue samples were collect 0,1,2,3,4, and 5 days after activation of the AP1-GR fusion protein. Samples of consecutive time points from each set were co-hybridized to micoarrays. Dyes used for labeling the RNA populations derived from the individual samples were switched in the replicate experiments to reduce dye-related artefacts.
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| Contributor(s) |
Wellmer F, Alves-Ferreira M, Dubois A, Riechmann J, Meyerowitz EM |
| Citation(s) |
15100403, 16789830 |
| |
| Submission date |
Apr 02, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Frank Wellmer |
| E-mail(s) |
[email protected]
|
| Phone |
626-395-8438
|
| Organization name |
California Institute of Technology
|
| Department |
Biology 156-29
|
| Lab |
Meyerowitz
|
| Street address |
1200 E. California Blvd.
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2810 |
Meyerowitz Lab Arabidopsis Operon Array Version 3 |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA94451 |
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