|
| Status |
Public on Jan 03, 2017 |
| Title |
Gene expression profiles of pre-confluent 3T3-L1 preadipocytes with low or high adipogenic potential |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The objective of this analysis was to identify the genes that are differentially expressed between preadipocytes with low or high adipogenic capability
|
| |
|
| Overall design |
Clones of 3T3-L1 were isolated by serial dilutions. Each well was visually inspected by microscopy to confirm the presence of a single colony per well. Wells with more than one colony were excluded. The clones were amplified and always maintained at sub-confluence. The adipogenic potential of each clonal lines was tested using an adipogenic cocktail containing insulin, dexamethasone and IBMX. After differentiation, triglycerides were extracted and quantified and clones with a low ability to differentiate into adipocytes were classified as a 'Low' lines and clones with a high adipogenic potential were classified as 'High' lines. For the microarray analysis, RNA was extracted from 5 Low lines and 5 High lines. The RNA was extracted prior to differentiation, in proliferating cells.
|
| |
|
| Contributor(s) |
Laplante M |
| Citation(s) |
28052263 |
| |
| Submission date |
Nov 28, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Mathieu Laplante |
| E-mail(s) |
[email protected]
|
| Organization name |
Université Laval
|
| Department |
Medicine
|
| Street address |
2725 Chemin Sainte-Foy
|
| City |
Québec |
| State/province |
Qc |
| ZIP/Postal code |
G1V4G5 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA355112 |
Less...
More...