|
| Status |
Public on Jan 03, 2017 |
| Title |
5C4_high adipogenic |
| Sample type |
RNA |
| |
|
| Source name |
pre-confluent 3T3-L1 cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pre-confluent 3T3-L1 cells
adipogenic potential: High
|
| Treatment protocol |
None
|
| Growth protocol |
Proliferating Low and High 3T3-L1 cells maintained in DMEM + 10% Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen, 74104)
|
| Label |
biotin
|
| Label protocol |
Total RNA was quantified using a NanoDrop Spectrophotometer ND-1000 (NanoDrop Technologies, Inc.) and its integrity was assessed using a 2100 Bioanalyzer (Agilent Technologies). Sense-strand cDNA was synthesized from 250 ng of total RNA, and fragmentation and labeling were performed to produce ss DNA with the Ambion® WT Expression Kit according to manufacturer’s instructions (Ambion).
|
| |
|
| Hybridization protocol |
After fragmentation and labeling, 3.5µg DNA target was hybridized on GeneChip® Mouse Gene 1.0 ST (Affymetrix) and incubated at 450C in the Genechip® Hybridization oven 640 (Affymetrix) for 17 hours at 60 rpm. GeneChips were then washed in a GeneChips® Fluidics Station 450 (Affymetrix) using Affymetrix Hybridization Wash and Stain kit according to the manufacturer’s instructions (Affymetrix).
|
| Scan protocol |
The microarrays were finally scanned on a GeneChip® scanner 3000 (Affymetrix).
|
| Data processing |
Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
|
| |
|
| Submission date |
Nov 28, 2016 |
| Last update date |
Jan 03, 2017 |
| Contact name |
Mathieu Laplante |
| E-mail(s) |
[email protected]
|
| Organization name |
Université Laval
|
| Department |
Medicine
|
| Street address |
2725 Chemin Sainte-Foy
|
| City |
Québec |
| State/province |
Qc |
| ZIP/Postal code |
G1V4G5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE90580 |
Gene expression profiles of pre-confluent 3T3-L1 preadipocytes with low or high adipogenic potential |
|
