|
| Status |
Public on Nov 26, 2013 |
| Title |
max2-3, well-watered (0h), replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
max2-3_well-watered
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype/variation: max2-3 mutant
age: 24 day-old
exposed to: 0h dehydration (well-watered)
tissue: rosette leaves
|
| Treatment protocol |
The aerial of 24-d-old plants were detached and exposed to dehydration on KimTowel papers for 0, 2 or 4h.
|
| Growth protocol |
Two-week-old WT and max2-3 mutant plants were transferred from GM plates to soil and grown for 10 additional day (22ºC, 16-h-light/8-h-dark, 60 μmol m-2 sec-1 photon flux density).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the rosette leaves using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instruction.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
1.65 µg of Cy3-labelled cRNA were fragmented at 60°C for 30 minutes following the manufacturer's instructions. Agilent hybridization buffer was then added to the fragmentation mixture and hybridized to Agilent 021169 Arabidopsis Ver.4 Oligo Microarray 4x44K G2519F for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarray slides were washed and dried immediately by a brief centrifugation.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44k array slides.
|
| Description |
max2-3-C_2
Gene expression data from well-watered max2-3
|
| Data processing |
The scanned images were analyzed with GeneSpring GX11.5.1 (Agilent). Percentile normalization (75 percentile) was performed for each chip.
|
| |
|
| Submission date |
Jul 17, 2013 |
| Last update date |
Nov 26, 2013 |
| Contact name |
chien Van ha |
| E-mail(s) |
[email protected]
|
| Organization name |
texas tech university
|
| Department |
IGCAST
|
| Lab |
Tran
|
| Street address |
1006 Carton Avenue, Box 43135
|
| City |
Lubbock |
| State/province |
TX |
| ZIP/Postal code |
79409 |
| Country |
USA |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE48949 |
Comparative expression analysis of the Arabidopsis strigolactone (SL)-response mutant, max2-3, and wild type (WT) plants (Col-0) under dehydration conditions. |
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