|
| Status |
Public on Jan 01, 2014 |
| Title |
empty vector transfected CD4+ T cells, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
cell type: CD4+ T cells
genotype/variation: empty vector transfected
|
| Treatment protocol |
24 hours after activation, cells were infected by retroviruses control empty vector (Empty-RV-GFP). One days after infection, GFP cells were FACS sorted.
|
| Growth protocol |
Naïve CD4+CD25-CD44lowCD62Lhigh T cells from C57BL6 mice were FACS-sorted and activated with plate-bound anti-CD3e (clone, 2C11) and anti-CD28 (clone, 37.51) under neutral conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
2 ug total RNA was labeled using Ambion® WT Expression Kit and GeneChip® WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Labeled RNA was hybrizized onto arrays using GeneChip® Hybridization, Wash, and Stain Kit
|
| Scan protocol |
GeneChip Operating Software (GCOS v1.1) was used.
|
| Description |
RV_1
|
| Data processing |
Data was summarized using RMA algorithm within GeneSpring 11.0.
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Nov 29, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Lai Wei |
| E-mail(s) |
[email protected]
|
| Phone |
3014961480
|
| Organization name |
NIH/NEI/NCCAM
|
| Street address |
10 Center Dr. Room 2B47
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE52838 |
Transcription factor Achaete-Scute homologue 2 initiates T follicular helper cell development [array] |
| GSE52840 |
Transcription factor Achaete-Scute homologue 2 initiates T follicular helper cell development |
|
