|
| Status |
Public on Jul 03, 2014 |
| Title |
GHTMM-miR133 |
| Sample type |
RNA |
| |
|
| Source name |
HCFs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HCFs (induced toward a cardiac-like phenotype (iCM))
treatment: GMTMM/miR-133 transduced
time: day 7
|
| Treatment protocol |
CSII-CMV lentivirus were used for transduction with Polybrene (Miilopore). Synthetic mimics of mature miRNA-133a (Thermo Scientific) were transfected simultaneously into cells with Lipofectamine 2000 (Invitrogen)
|
| Growth protocol |
24 h after transduction, the medium was replaced with D-MEM (high glucose) with L-Glutamate and Phenol Red (Wako, 044-29765) /Medium199 with Earle’s Salts, L-Glutamate and 22g/L Sodium Bicarbonate (Gibco, 11150-059)/10% Hyclone Characterized FBS (Thermo Scientific, SV30014.03) medium and changed every 2-3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using ReliaPrep™ RNA Cell Miniprep System (Promega).
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
GMTMM/miR-133 transduced-HCFs
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots' signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Apr 17, 2014 |
| Last update date |
Jul 03, 2014 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (2) |
| GSE56874 |
Gene expression profile of human cardiac fibroblasts (HCFs), induced cardiomyocytes (iCMs), and heart |
| GSE56913 |
Gene expression profiles of induced cardiomyocytes(iCMs) |
|
