|
| Status |
Public on Feb 15, 2019 |
| Title |
SREBP1-siRNA_serum-free_normoxia |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer cell line, OVSAYO
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
|
| Treatment protocol |
OVSAYO cells were transfected with non-specific (Silencer negative control siRNA (Ambion)) or SREBP1 (SMRT pool (Dharmacon)) siRNA using lipofectamine 2000 (Life technologies, Carlsbad, CA,USA) and cultured for 30 h in RPMI1640 medium (10% fetal calf serum). Cells were washed once with serum-free medium. Cells were further cultured under serum-free condition for 16 h under normoxia and hypoxia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using SV total RNA isolation system (Promega).
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
gene expression in cells transfected with SREBP1-siRNA, and then cultured under serum-free and normoxia condition
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Feb 14, 2018 |
| Last update date |
Feb 15, 2019 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE110615 |
Genes activated in an SREBP1 dependent manner in ovarian cancer cells exposed to serum starvation and hypoxia |
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