Total RNA was extracted and purified using QIAamp RNA Blood Mini Kit（Cat#52304, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Label
biotin
Label protocol
Biotinylated cDNA were prepared according to NUGEN Ovation® WB Reagent( 1300, NUGEN )and Encore® Biotin Module( 4200, NUGEN )’s instructions from 100 ng total RNA
Hybridization protocol
GeneChips were washed and stained using GeneChip® HT Hybridization, Wash, and Stain Kit (901405 ,Affymetrix)
Scan protocol
GeneChips were scanned using the GeneTitan system.
Description
Expression data of peripheral blood leukocytes in patients with Health
