|
| Status |
Public on Mar 06, 2010 |
| Title |
Adult_Capsule_3 |
| Sample type |
RNA |
| |
|
| Source name |
renal capsule
|
| Organism |
Mus musculus |
| Characteristics |
gene reported: wild-type
strain: CD-1
Sex: male
developmental stage: Adult
theiler stage: TS28
somite count: n/a
developmental landmark: n/a
cell type: capsule cell
|
| Treatment protocol |
pool size: 1 Adult kidney
Pooled sample: No
Dissection Method: Microdissection
Adult CD-1 mice were euthanized by standard carbon dioxide asphyxiation. Kidneys were removed in ice-cold PBS. Using microforceps, the capsule of the kidney was removed and place into an eppendorf tube for RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Potter protocol: 'RNA purification'
|
| Label |
Biotin
|
| Label protocol |
The FL-Ovation cDNA Biotin Module V2 (NuGEN) is used to chemically modify fragments and label target sense cDNA.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
Amount labeled target hybridization to array: 2.5µg
The arrays were washed and stained using a Fluidics Station 450 (Affymetrix) utilizing the fluidics protocol FS450-0007.
|
| Scan protocol |
Probe arrays were scanned using an Affymetrix GeneChip Scanner 3000 7G and Affymetrix scanning software Genechip Operating Software Version 1.4.
|
| Description |
>> Amplification protocol <<
Target Amplified manufacturer/kit: WT-Ovation Pico RNA Amplification System (NuGEN)
Target Amplified protocol: Potter protocols
Rounds of amplification: 1
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:13519
|
| Data processing |
Analysis method: Affymetrix Expression Console and GeneSpring 10.
|
| |
|
| Submission date |
Mar 05, 2010 |
| Last update date |
Dec 29, 2010 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
[email protected]
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE20639 |
Gene expression profiles of adult renal capsule isolated from kidneys using micro-dissection. (GUDMAP Series ID: 36) |
|
