|
| Status |
Public on Oct 19, 2010 |
| Title |
LiPS-45-3 |
| Sample type |
RNA |
| |
|
| Source name |
inducible pluripotent stem cell derived from mouse embryonic fibroblast using 3 factors
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: inducible pluripotent stem cell
protocol: with tetraploid embryo complementation competence
|
| Growth protocol |
cultured in embryonic stem cell culture medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
following manufacturer’s recommendation using Trizol reagent (Invitrogen) WT cDNA Synthesis and Amplification Kit(Affymetrix, Inc.)
|
| Label |
biotin
|
| Label protocol |
WT Terminal Labeling Kit (Affymetrix, Inc.)
|
| |
|
| Hybridization protocol |
Hybridization, Wash and Stain Kit(Affymetrix, Inc.)
|
| Scan protocol |
GeneChip® Scanner 3000 7G3000 (Affymetrix, Inc.)
|
| Description |
biological repeat 3
|
| Data processing |
GCOS 1.4 software provided by Affymetrix
|
| |
|
| Submission date |
Sep 09, 2010 |
| Last update date |
Oct 19, 2010 |
| Contact name |
Lan Kang |
| Organization name |
Dalian Medical University
|
| Department |
Institute of Cancer Stem Cell
|
| Street address |
9 West Section, Lvshun South Road
|
| City |
Dalian |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE24046 |
Viable Mice Produced from 3-factor Induced Pluripoent Stem (iPS) Cells through Tetraploid Complementation |
|
