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| Status |
Public on Oct 19, 2010 |
| Title |
Viable Mice Produced from 3-factor Induced Pluripoent Stem (iPS) Cells through Tetraploid Complementation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Ectopic expression of four transcription factors including Oct4, Sox2, Klf4 and c-Myc in differentiated fibroblast cells could reset the cell fate of fibroblast cells to pluripotent state. Subsequently, fully pluripotency of these so-called induced pluripotent stem cells (iPSCs) has been demonstrated as viable mice could be generated autonomously from iPS cells through tetraploid blastocyst complementation. Moreover, the generation of human and patient-specific iPS cells have raised the possibility of utilizing iPS cells clinically. However, the utilization of c-Myc in iPS cells induction greatly increased the incidence of tumorigenecity in the iPS-chimeric mice and also might hinder the clinical application of human iPS cells in the future. Fortunately, c-Myc has been recently found dispensable for iPS induction even though the iPS induction efficiency is greatly reduced in the absence of c-Myc. However, it remains unknown if these three factors-induced iPS cells are fully pluripotent. In the present study, we have successfully demonstrated that 3-factor iPS cells could also be fully pluripotent as viable mice could be generated from 3-factor iPS cells autonomously via tetraploid complementation and moreover, our data indicated that the pluripotency regulatory mechanism in 3-factor iPS cells might be distinct from 4-factor iPS cells.
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| Overall design |
We compared the gene expression profile of iPS cells with and without the tetraploid embryo complementation competence. Three biological repeats were included for each line.
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| Contributor(s) |
Kang L, Wu T, Tao Y, Yuan Y, He J, Zhang Y, Luo T, Kou Z, Gao S |
| Citation(s) |
21119684 |
| |
| Submission date |
Sep 09, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Lan Kang |
| Organization name |
Dalian Medical University
|
| Department |
Institute of Cancer Stem Cell
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| Street address |
9 West Section, Lvshun South Road
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| City |
Dalian |
| ZIP/Postal code |
116044 |
| Country |
China |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA130349 |
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