|
Status |
Public on Oct 19, 2010 |
Title |
LiPS-47-1 |
Sample type |
RNA |
|
|
Source name |
inducible pluripotent stem cell derived from mouse embryonic fibroblast using 3 factors
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J cell type: inducible pluripotent stem cell protocol: without tetraploid embryo complementation competence
|
Growth protocol |
cultured in embryonic stem cell culture medium
|
Extracted molecule |
total RNA |
Extraction protocol |
following manufacturer’s recommendation using Trizol reagent (Invitrogen) WT cDNA Synthesis and Amplification Kit(Affymetrix, Inc.)
|
Label |
biotin
|
Label protocol |
WT Terminal Labeling Kit (Affymetrix, Inc.)
|
|
|
Hybridization protocol |
Hybridization, Wash and Stain Kit(Affymetrix, Inc.)
|
Scan protocol |
GeneChip® Scanner 3000 7G3000 (Affymetrix, Inc.)
|
Description |
biological repeat 1
|
Data processing |
GCOS 1.4 software provided by Affymetrix
|
|
|
Submission date |
Sep 09, 2010 |
Last update date |
Oct 19, 2010 |
Contact name |
Lan Kang |
Organization name |
Dalian Medical University
|
Department |
Institute of Cancer Stem Cell
|
Street address |
9 West Section, Lvshun South Road
|
City |
Dalian |
ZIP/Postal code |
116044 |
Country |
China |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE24046 |
Viable Mice Produced from 3-factor Induced Pluripoent Stem (iPS) Cells through Tetraploid Complementation |
|