|
| Status |
Public on Nov 13, 2010 |
| Title |
Lung_Uninfected-1_28d |
| Sample type |
RNA |
| |
|
| Source name |
mouse lung, Uninfected, 28d
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
disease state: uninfected
diagnosis: untreated
collection date: 28
|
| Treatment protocol |
Mice were treated with CC-3052 (PDE4 inhibitor, 25mg/kg/day) suspended in 0.2% carboxymethylcellulose solution by Ad libitum 5 days a week.
|
| Growth protocol |
mouse lungs were collected after 14, 21, 28 and 42 days after Mtb CDC1551 infection (low aerosol exposure, 200 cfu) with or without CC-3052 treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction was performed to isolate total RNA according to manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
300 ng of total RNA was processed using the GeneChip WT labelling protocol, version 4 cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Followng fragmentation, labeled single stranded DNA hybrized unto the Mouse Gene 1.0 ST array, and placed in 45°C hybridization oven, at 60 rpm, and incubated for 17± 1 hours
|
| Scan protocol |
Gene chips were scanned using an Affymetrix Genechip Scanner 3000 7G Plus
|
| Data processing |
GeneChip CEL files were analyzed using Partek Genomics Suite
|
| |
|
| Submission date |
Nov 12, 2010 |
| Last update date |
Nov 12, 2010 |
| Contact name |
Mi-Sun Koo |
| E-mail(s) |
[email protected]
|
| Phone |
9738543226
|
| Fax |
972854322
|
| Organization name |
University of Medicine and Dentistry of New Jersey
|
| Department |
Public Health Research Institute
|
| Street address |
225 Warren Street, W250Q
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25313 |
Effect of PDE4 inhibition on host gene expression of Mtb-infected mouse lungs. |
|
