|
| Status |
Public on Dec 18, 2011 |
| Title |
DMSO_batch2_e [Affy] |
| Sample type |
RNA |
| |
|
| Source name |
Terminally differentiated Neurons (TN)
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129Sv-C57Bl/6
cell type: Terminally differentiated Neurons (TN)
agent: DMSO
agent batch: 2
protocol: control
biological replicate: e
|
| Treatment protocol |
Cells were treated with either DMSO or 25 uM of the drug three times at an interval of 2 hours
|
| Growth protocol |
Cells were grown at 37°C cell culture incubator in complete media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cells using Rneasy kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
RNA was processed according to the GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual from Affymetrix
|
| |
|
| Hybridization protocol |
hybridization was performed according to standard Affymetrix procedure
|
| Scan protocol |
Scanning was performed with Affymetrix GCC Scan Control v. 3.0.0.1214
|
| Data processing |
probesets were summarized and probeset-level values normalized with rma (target=core) function from R/Bioconductor package oligo (version 1.14.0)
|
| |
|
| Submission date |
Nov 22, 2010 |
| Last update date |
Dec 22, 2011 |
| Contact name |
Dirk Schuebeler |
| Organization name |
Friedrich Miescher Institute for Biomedical Research
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE25529 |
Expression data from DMSO and SP600125 treated neurons |
| GSE25533 |
A chromatin-modifying function of JNK during embryonic stem cell differentiation |
|
