|
| Status |
Public on Dec 01, 2011 |
| Title |
quiescent p27WT MEFs 2 |
| Sample type |
RNA |
| |
|
| Source name |
WT
|
| Organism |
Mus musculus |
| Characteristics |
cell type: MEFs
genotype/variation: wild type
|
| Treatment protocol |
For serum starvation, cells were maintained without serum during 72 h.
|
| Growth protocol |
Wt, CK and p27KO MEFs were cultured in DMEM supplemented with 10% fetal bovin serum (FBS). All cultures were maintained at 37ºC and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Kit (QIagen). RNAs from quiescent MEFs from wt or delta51 mice were analyzed using a Bioanalyzer (Agilent). Only samples with RIN number greater than 9 and a ratio A260/280 over than 2 were further used in microarray analysis. Retrotranscription was made followed by labeling with biotin.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45ºC on Affymetrix 1.0 ST murine array. Chips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
Arrays were scanned using the Scanner Axon 4000B
|
| Description |
Gene expression data from WT MEFs cells
|
| Data processing |
The normalization of the arrays was done using the rma method as implemented in the Affy package of R Bioconductor. Quality control measurements were done using ArrayQualityMetrics package of R Bioconductor.
|
| |
|
| Submission date |
Mar 03, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
oriol bachs |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
636574156
|
| Fax |
0034-934021907
|
| Organization name |
University of Barcelona
|
| Department |
Cell Biology, Immunology and Neurosciences
|
| Street address |
Casanova 143
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE20069 |
p27Kip1 regulates transcription of genes involved in tumorigenesis |
| GSE27672 |
Expression data from p27WT, p27CK and KO MEFs cells in quiescence |
|
