|
| Status |
Public on Dec 15, 2011 |
| Title |
IKK2 WT cell line, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
IKK2 WT cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell type: tumor-derived cell line
ikk2 status: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was harvested with Trizol according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
total RNA was labeled with the Ambion Affymetrix WT expression kit for Affymetrix whole transcript arrays, product #4411974. Product number 4411974
|
| |
|
| Hybridization protocol |
Fragmented cRNA are hybridized using a GeneChip Fluidics Station 450 in aGeneChip Hybridization Oven 640.
|
| Scan protocol |
Gene chips were scanned using an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
cell lines were subcloned from WT and IKK2-/- primary tumors by serial dilution
|
| Data processing |
Gene chips were analyzed using affymerix power tools using MAS 5 background correction, RMA probeset summarization, and median normalization (-a mas5-bg,med-norm,pm-only,med-polish).
|
| |
|
| Submission date |
Jun 17, 2011 |
| Last update date |
Dec 17, 2011 |
| Contact name |
Eugene Ke |
| Organization name |
University of Virginia
|
| Department |
MIC
|
| Street address |
P.O. Box 800734, 1340 Jefferson Park Avenue
|
| City |
Charlottesville |
| State/province |
Virginia |
| ZIP/Postal code |
22903 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30049 |
Expression data comparing the effect of IKK2 KO on a KRAS mouse model |
|
