GEO DataSets

Expression profiling of estrogen receptor positive breast cancer cell lines treated with estradiol for 24 hours. MCF-7, T47-D, and BT-474 breast cancer cell lines examined. Results identify candidate genes involved in estrogen stimulated breast cancer growth.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 cell line sets

Platform:

GPL96

Series:

GSE3529

12 Samples

Download data: CEL

(Submitter supplied) Three human ER+ breast cancer cell lines--MCF-7, T47-D, BT-474--grown with or without estradiol (E2). Keywords: Cell Line Comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1549

Platform:

GPL96

12 Samples

Download data: CEL

(Submitter supplied) Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1326

Platform:

GPL96

12 Samples

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Analysis of the response of estrogen receptor (ER) negative MDA-MB-231 breast cancer cells infected with full-length ER alpha adenoviral constructs to treatment with 17beta-estradiol (E2). Results provide insight into the anti-proliferative effect of E2 on breast cancer cells reexpressing ER.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:

GPL96

Series:

GSE2251

12 Samples

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(Submitter supplied) Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

4 Samples

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(Submitter supplied) Alternative promoters (APs) occur in >30% protein-coding genes and contribute to proteome diversity. However, large-scale analyses of AP regulation are lacking, and little is known about their potential physiopathologic significance. To better understand the transcriptomic impact of estrogens, which play a major role in breast cancer, we analyzed gene and AP regulation by estradiol in MCF7 cells using pan-genomic exon arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL5175 GPL5188

32 Samples

Download data: CEL

(Submitter supplied) We generated DNA microarray based gene expression profiles from three estrogen receptor a (ERa) positive breast cancer cell lines stimulated by 17ß-estradiol (E2) in vitro over a time course, as well as from MCF-7 cells grown as xenografts in ovariectomized athymic nude mice with E2 supplementation and after its withdrawal. Keywords: Cell line and xenograft comparisons

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL96

47 Samples

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(Submitter supplied) To obtain comprehensive information on 17beta-estradiol (E2) sensitivity of genes that are inducible or suppressible by this hormone, we designed a method that determines ligand sensitivities of large numbers of genes using DNA microarray and a set of simple Perl computer scripts implementing the standard metric statistics, and employed it to characterize effects of low (0-100 pM) concentrations of E2 on the transcriptome profile of MCF7/BUS human breast cancer cells, whose E2 dose-dependent growth curve saturated with 100 pM E2. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2323 GDS2324

Platform:

GPL96

34 Samples

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Analysis of breast cancer MCF7/BUS cells treated with 17beta-estradiol (E2) at concentrations up to 100 pM. Results compared to that from an experiment examining the response to hormone starvation (GDS2323). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 dose sets

Platform:

GPL96

Series:

GSE4668

25 Samples

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Analysis of breast cancer MCF7/BUS cells starved of estrogen for up to 2 days. Results compared to that from an experiment (GDS2324) examining the response to low concentrations of 17beta-estradiol (E2). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 time sets

Platform:

GPL96

Series:

GSE4668

9 Samples

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(Submitter supplied) Several genome-wide transcriptome analyses that focused on p53-induced cellular responses in many cellular contexts have continued to expand the already vast p53-regulated transcriptional networks. To investigate post-transcriptional controls as an additional dimension of p53-directed gene expression responses we performed translatome analysis by polysomal profiling on MCF7 cells treated with Doxorubicin and Nutlin-3a. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

27 Samples

Download data: TXT

(Submitter supplied) We explored the combinatorial interactions between p53, estrogen receptors and NFkB using the breast adenocarcinoma-derived MCF7 cells. Recently, we have established that p53 can modulate transcription also through non-canonical response elements (REs), consisting of half-sites and ¾ sites. In particular, we identified a half-site p53 response element in the promoter of FLT1/VEGFR-I gene that was required but not sufficient for the p53 responsiveness of the promoter. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

40 Samples

Download data: TXT

(Submitter supplied) Methods for identifying protein-protein interactions have mostly been limited to tagged exogenous expression approaches. We now establish a rapid, robust and comprehensive method for finding interacting proteins using endogenous proteins from limited cell numbers. We apply this approach called ‘Rapid IP-Mass Spectrometry of Endogenous proteins (RIME)’ to identify ER, FoxA1 and E2F4 interacting proteins in breast cancer cells. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL10999 GPL11154

16 Samples

Download data: BED

(Submitter supplied) Methods for identifying protein-protein interactions have mostly been limited to tagged exogenous expression approaches. We now establish a rapid, robust and comprehensive method for finding interacting proteins using endogenous proteins from limited cell numbers. We apply this approach called ‘Rapid IP-Mass Spectrometry of Endogenous proteins (RIME)’ to identify ER, FoxA1 and E2F4 interacting proteins in breast cancer cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

23 Samples

Download data: TXT

(Submitter supplied) Estrogen Receptor-alpha (ER) is the key driver of 75% of all breast cancers. Upon stimulation by its ligand estra-2-diol, ER forms a transcriptionally active complex binding chromatin. Previous studies have reported that ER binding follows a cyclical binding pattern with a periodicity of 90 minutes. However, these studies have been limited to individual ER target genes and most were done without replicates. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20301

59 Samples

Download data: CSV

(Submitter supplied) Changes in the expression of 8400 genes were monitored by cDNA microarray analysis during the first 32 h of human breast cancer (BC) ZR-75.1 cell stimulation with a mitogenic dose of 17beta-estradiol, a timing which corresponds to completion of a full mitotic cycle in hormone-stimulated cells. Hierarchical clustering of 344 genes whose expression either increases or decreases significantly in response to estrogen reveals that the gene expression program activated by the hormone in these cells shows 8 main patterns of gene activation/inhibition. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL970

25 Samples

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(Submitter supplied) We performed the single-cell analysis to characterize estrogen regulation in ER-positive breast cancer using patient-derived xenograft (PDX) model (named SC31).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

2 Samples

Download data: MTX, TSV

(Submitter supplied) We performed RNA sequencing analysis to see gene expression changes after estrogen treatment for an aromatase inhibitor-resistant PDX model (named GS3).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

13 Samples

Download data: TXT

(Submitter supplied) We performed the single-cell analysis to reveal the mechanism of estrogen-induced cell cycle arrest using an Aromatase inhibitor-resistant patient-derived xenograft model (named GS3).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: MTX, TSV

(Submitter supplied) Using microarray analysis, we identified a unique ras superfamily gene, termed RERG (ras-related and estrogen-regulated growth inhibitor), whose expression was decreased or lost in a significant percentage of primary human breast tumors that show a poor clinical prognosis. Importantly, high RERG expression correlated with expression of a set of genes that define a breast tumor subtype that is estrogen receptor-positive and associated with a slow rate of tumor cell proliferation and a favorable prognosis for these cancer patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL2671 GPL2669 GPL2670

5 Samples

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