GEO DataSets

(Submitter supplied) We used a combination of adherence assays, mutagenesis and functional genomics to identify novel factors involved in adherence. This work identifies a list of novel potential pneumococcal adherence determinants.

Organism:

Streptococcus pneumoniae TIGR4

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16764

1 Sample

Download data: TXT

(Submitter supplied) Infection of the human host by Streptococcus pneumoniae begins with colonization of the nasopharynx, which is mediated by adherence of bacteria to respiratory epithelium. Several studies have indicated an important role for the pneumococcal capsule in this process. Here, we used microarrays to characterize the in vitro transcriptional response of human nasopharyngeal epithelial Detroit 562 cells to adherence of serotype 2-encapsulated strain D39, serotype 19F-encapsulated strain G54, serotype 4-encapsulated strain TIGR4, and their nonencapsulated derivatives (Δcps). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3041

Platform:

GPL570

32 Samples

Download data: CEL, EXP

Analysis of pharyngeal epithelial cells exposed to various encapsulated strains of Streptococcus pneumoniae. Nasopharynx colonization by S. pneumoniae is mediated by adherence to the respiratory epithelium. Results provide insight into the capsule-dependent host response to pneumococcal adherence.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 agent, 3 cell type, 3 dose, 2 infection sets

Platform:

GPL570

Series:

GSE8527

32 Samples

Download data: CEL, EXP

(Submitter supplied) In order to elucidate the regulatory role of pneumococcal TCS08, a transcriptomic approach was performed using microarray from bacterial cultures in chemical defined medium of single and double tcs08 mutants. A total of 159 genes presenting significant expression changes were selected for further evaluation and discussion. These 159 genes were classified accordingly to their biochemical function.

Organism:

Streptococcus pneumoniae; Streptococcus pneumoniae TIGR4

Type:

Expression profiling by array

Platform:

GPL24482

12 Samples

Download data: TXT, XLSX

(Submitter supplied) The objective of the study is to obtain a high resolution transcription map of S. pneumoniae genome and make it available through a genome browser. The study also help to identify gene expression pattern, presence of small RNAs, Antisense expression and operon structure.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by genome tiling array

Platform:

GPL7177

2 Samples

Download data: PAIR

(Submitter supplied) This microarray experiment is part of a study addressing the importance of glutamine metabolism in Streptococcus pneumoniae for the virulence of this bacterium. To be able to gain more insight into the phenotype of a double mutant of glnA (TIGR 4 locus tag SP0502, encoding glutamine synthetase) and glnP (SP1241, encoding a permease component of a glutmine uptake ABC transporter), the transcriptome of this mutant, the glnPA double mutant, was determined in strain S. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL6268

4 Samples

Download data: TXT

(Submitter supplied) For Streptococcus pneumoniae, biofilms have been suggested to promote long-term colonization of the nasopharynx and contribute to the pathology of recurrent middle ear infections. To date numerous studies have investigated the contribution of specific genetic determinants for the development of pneumococcal biofilms, however, studies examining the global changes that occur during biofilm development and how they contribute to disease are lacking. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL10220

4 Samples

Download data: TAV

(Submitter supplied) Recent murine studies have demonstrated that the role of response regulator 09 (RR09) of S. pneumoniae in virulence varies between strains. In the present study, we used a murine pneumonia model of infection to assess the virulence of a TIGR4 rr09-mutant, and found that TIGR4Δrr09 was attenuated after intranasal infection. Further, we investigated the in vitro transcriptional changes in pneumococcal rr09 mutants of two strains, D39 and TIGR4, by microarray analysis. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL4681

13 Samples

Download data

(Submitter supplied) CodY is a nutritional regulator mainly involved in amino acid metabolism. It has been extensively studied in Bacillis subtilis and Lactococcus lactis. We investigated the role of CodY in gene regulation and virulence of the human pathogen Streptococcus pneumoniae. We constructed a codY-mutant and examined the effect on gene and protein expression by microarray and 2D DIGE analysis. The pneumococcal CodY-regulon was found to consist predominantly of genes involved in amino acid metabolism, but also several other cellular processes, such as carbon metabolism and iron uptake. more...

Organism:

Streptococcus pneumoniae TIGR4; Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL5023

7 Samples

Download data: TXT

(Submitter supplied) Previous studies have indicated that PsaR of Streptococcus pneumoniae is a manganese-dependent regulator, negatively affecting the expression of at least seven genes. Here, we extended these observations by transcriptome and proteome analysis of psaR mutants in strains D39 and TIGR4. The microarray analysis identified three shared PsaR targets: the psa-operon, pcpA, and prtA. Additionally, we found 31 genes to be regulated by PsaR in D39 only, most strikingly a cellobiose-specific PTS and a putative bacteriocin operon (sp0141-sp0146). more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL6268

6 Samples

Download data: TXT

(Submitter supplied) We were interested in determining if the LuxS signaling system contributed to the regulation of pneumococcal genes. To accomplish this, we compared the in vitro transcriptional profiles over time of S. pneumoniae D39, a serotype 2 strain of pneumococcus, with that of an isogenic deletion mutant, (delta)luxS, using a spotted S. pneumoniae- specific DNA microarray. For in vitro time course experiments, broth cultures of D39 and (delta)luxS were grown to low density (optical densities between ~0.01- 0.02), the cells were collected by centrifugation, washed 1x with sterile PBS, back diluted to ~3x105CFU/ ml into fresh broth, and immediately incubated at 37oC in a 5% CO2 chamber for 45 minutes. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL2712

40 Samples

Download data

(Submitter supplied) The primary mechanism by which pneumococcal capsular polysaccharide-based vaccines are believed to mediate protection is by induction of serotype-specific opsonic antibodies that facilitate bacterial killing by phagocytes (opsonophagocytosis). However, antibodies that are protective against experimental pneumococcal pneumonia in mice but do not promote opsonophagocytic killing in vitro have also been identified 1-3. more...

Organism:

Streptococcus pneumoniae TIGR4; Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL13163

6 Samples

Download data: CALLS, PAIR

(Submitter supplied) Previous studies indicate that host phagocytes are regulated by a complex combination of pattern recognition receptors-signaling and miRNA inductions. We performed microRNA array to determine whether S. pneumoniae PfbA suppresses phagocytosis via miRNA inductions.

Organism:

synthetic construct; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

5 Samples

Download data: CEL