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Links from GEO DataSets

Items: 20

1.

Highly efficient generation of blastocyst-like structures from mouse totipotent blastomere-like cells

(Submitter supplied) Mammalian embryogenesis begins with a totipotent zygote. Early embryogenesis can be recapitulated by aggregated extended pluripotent stem cells (EPSCs) in a 3D culture system. However, the efficiency of generating blastoids is low and whether other reported totipotent stem cells retained a similar capacity remains unknown. Here we show that spliceosomal repressed totipotent blastomere-like cells (TBLCs) form blastocyst-like structures when aggregated in 3D microwells with around 80% efficiency. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL17021 GPL24247
8 Samples
Download data: TXT
Series
Accession:
GSE197779
ID:
200197779
2.

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells (single-cell RNA-Seq)

(Submitter supplied) A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
2 Samples
Download data: MTX, TSV
Series
Accession:
GSE135701
ID:
200135701
3.

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells

(Submitter supplied) A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21273
8 Samples
Download data: TXT
Series
Accession:
GSE135289
ID:
200135289
4.

Capturing totipotency in human cells through spliceosomal repression

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL20795
112 Samples
Download data: BW, NARROWPEAK, TXT
Series
Accession:
GSE226231
ID:
200226231
5.

Capturing totipotency in human cells through spliceosomal repression [ATAC-seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Assay for transposase accessible chromatin with high-throughput sequencing in hESC sand hTBLCs
Organism:
Homo sapiens
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL20795
2 Samples
Download data: NARROWPEAK
Series
Accession:
GSE226229
ID:
200226229
6.

Capturing totipotency in human cells through spliceosomal repression [scRNA-seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20795
14 Samples
Download data: TXT
Series
Accession:
GSE226228
ID:
200226228
7.

Capturing totipotency in human cells through spliceosomal repression [ChIP-seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for h3k4me3 and h3k27me3 in hESC sand hTBLCs
Organism:
Homo sapiens
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL20795
4 Samples
Download data: BW
Series
Accession:
GSE226227
ID:
200226227
8.

Capturing totipotency in human cells through spliceosomal repression [RNA-Seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cellb blastomeres Poly(A) RNA-seq is performed on hESCs cultured in MYCP medium
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL20795
92 Samples
Download data: CSV
Series
Accession:
GSE224794
ID:
200224794
9.

Mouse totipotent stem cells captured and maintained through spliceosomal repression

(Submitter supplied) Since the establishment of the first embryonic stem cells (ESCs), in vitro culture of totipotent cells functionally and molecularly comparable to in vivo blastomeres with embryonic and extraembryonic developmental potency is unviable. Spliceosomes are responsible for mRNA splicing and maturation. Here, we report that spliceosomal repression in mouse ESCs drives pluripotent-to-totipotent state transition. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing
Platforms:
GPL24247 GPL21273 GPL17021
50 Samples
Download data: COV, CSV, NARROWPEAK
Series
Accession:
GSE168728
ID:
200168728
10.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
9 Samples
Download data: TAB
Series
Accession:
GSE200935
ID:
200200935
11.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates [scRNA-seq]

(Submitter supplied) Our study provides an alternative strategy to generate human blastoids and model early embryogenesis up to pre-gastrulation stage, facilitating the insights into human embryonic development
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
1 Sample
Download data: TAB
Series
Accession:
GSE200934
ID:
200200934
12.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates [RNA-seq]

(Submitter supplied) Our study provides an alternative strategy to generate human blastoids and model early embryogenesis up to pre-gastrulation stage, facilitating the insights into human embryonic development
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
8 Samples
Download data: TXT
Series
Accession:
GSE200933
ID:
200200933
13.

Employing totipotent cells to reconstruct embryogenesis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21273
13 Samples
Download data: MTX, TSV
Series
Accession:
GSE232718
ID:
200232718
14.

Employing totipotent cells to reconstruct embryogenesis [scRNA-seq]

(Submitter supplied) Blastoids, a structure similar to blastocysts in morphological and molecular level, can be applied to regeneration research. Using totipotent cells to construct blastoids will extend the information of early development to an earlier stage, and explore clues of regeneration. Totipotent blastomere-like cells (TBLCs) are a novel type of stably cultured mouse totipotent cell line generated by inhibiting spliceosomes. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21273
4 Samples
Download data: MTX, TSV
Series
Accession:
GSE232715
ID:
200232715
15.

Employing totipotent cells to reconstruct embryogenesis [RNA-seq]

(Submitter supplied) Blastoids, a structure similar to blastocysts in morphological and molecular level, can be applied to regeneration research. Using totipotent cells to construct blastoids will extend the information of early development to an earlier stage, and explore clues of regeneration. Totipotent blastomere-like cells (TBLCs) are a novel type of stably cultured mouse totipotent cell line generated by inhibiting spliceosomes. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21273
9 Samples
Download data: XLSX
Series
Accession:
GSE232634
ID:
200232634
16.

Generation of blastocyst-like structures from human embryonic stem cells III [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
4 Samples
Download data: CSV
Series
Accession:
GSE163445
ID:
200163445
17.

Generation of blastocyst-like structures from human embryonic stem cells II [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
4 Samples
Download data: CSV
Series
Accession:
GSE163423
ID:
200163423
18.

Generation of blastocyst-like structures from human embryonic stem cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
9 Samples
Download data: CSV
Series
Accession:
GSE150578
ID:
200150578
19.

Generation of blastocyst-like structures from human embryonic stem cells I [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
1 Sample
Download data: CSV
Series
Accession:
GSE150570
ID:
200150570
20.

Human blastoids model blastocyst development and implantation [Bulk RNA-seq]

(Submitter supplied) Shortly after fertilization, human embryos implant into the uterus. This requires the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Appropriate stem cells can form a blastocyst model, which we termed blastoid. Here we show that naive human pluripotent stem cells (hPSCs) triply inhibited for the Hippo, TGF-β, and ERK pathways consistently and efficiently (>70%) form blastoids that generate transcriptional pre-implantation analogs of the three founding lineages (trophoblast, epiblast, primitive endoderm; >97%) according to the  sequence and pace of blastocyst development. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
61 Samples
Download data: TXT
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