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| Status |
Public on Sep 03, 2024 |
| Title |
Pseudomonas aeruginosa post-translational responses to elevated c-di-GMP levels |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
C-di-GMP signaling can directly influence bacterial behavior by affecting the functionality of c-di-GMP-binding proteins. In addition, c-di-GMP can exert an indirect and more global effect on gene transcription or translation, e.g. via riboswitches or by binding to transcription factors. In this study, we investigated the effects of changes in intracellular c-di-GMP levels on gene expression and protein production in the opportunistic pathogen Pseudomonas aeruginosa. We induced c-di-GMP production via an ectopically introduced diguanylate cyclase and recorded the transcriptional, translational as well as proteomic profile of the cells. We demonstrate that rising levels of c-di-GMP in P. aeruginosa under growth conditions otherwise characterized by low c-di-GMP levels immediately cause a switch to a non-motile, auto-aggregative phenotype. This switch became apparent before any c-di-GMP-dependent role on transcription, translation, or protein abundance could be observed. Our results indicate that sudden rises in global c-di-GMP pools affect the P. aeruginosa phenotype via an alteration of protein functionality, rather than an impact on global gene transcription or translation.
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| Overall design |
Profiling of cellular consequences of an elevated c-di-GMP level in exponential phase – RNA-seq, Ribo-seq
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| Contributor(s) |
Bense S, Witte J, Preusse M, Koska M, Pezoldt L, Dröge A, Hartmann O, Müsken M, Schulze J, Fiebig T, Bähre H, felgner S, Pich A, Häussler S |
| Citation(s) |
35362616 |
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| Submission date |
Sep 03, 2021 |
| Last update date |
Dec 03, 2024 |
| Contact name |
Matthias Preusse |
| Organization name |
Helmholtz Centre for Infection Research
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| Street address |
Inhoffenstr. 7
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| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
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| Platforms (2) |
| GPL18782 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1) |
| GPL28916 |
Illumina NovaSeq 6000 (Pseudomonas aeruginosa PAO1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA760480 |
| SRA |
SRP335571 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE183394_rpg_table.txt.gz |
95.6 Kb |
(ftp)(http) |
TXT |
| GSE183394_rpg_table2.csv.gz |
56.7 Kb |
(ftp)(http) |
CSV |
| GSE183394_rpg_table_RNASeq_repetition.txt.gz |
88.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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