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| Status |
Public on Mar 06, 2010 |
| Title |
Expression analysis of AOM-induced tumors and serrated tumors in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Abstract: Colonic cancers with a serrated morphology have been proposed to comprise a molecularly distinct tumor entity following an alternative pathway of genetic alterations independently of APC mutations. Here we demonstrate that intestinal cell specific expression of oncogenic K-rasG12D in mice induces serrated hyperplasia, which is characterized by p16ink4a overexpression and induction of senescence. Deletion of Ink4a/Arf in K-rasG12D expressing mice prevents senescence and leads to invasive, metastasizing carcinomas with morphological and molecular alterations comparable to human KRAS mutated serrated tumors. Thus, we suggest that oncogenic K-ras is sufficient to initiate an alternative, serrated pathway to colorectal cancer and hence propose RAS-RAF-MEK signaling apart from APC as an additional gatekeeper in colorectal tumor development.
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| |
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| Overall design |
2 to 3 tissue samples for each genotype were analyzed
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| |
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| Contributor(s) |
Bennecke M, Greten FR |
| Citation(s) |
20708155 |
| |
| Submission date |
Mar 05, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Moritz Bennecke |
| Organization name |
Klinikum r.d.Isar
|
| Department |
II. Med Klinik
|
| Lab |
F.R. Greten
|
| Street address |
Ismaninger Str. 22
|
| City |
Muenchen |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA124871 |
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