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| Status |
Public on Jan 27, 2004 |
| Title |
Effects of cigarette smoke on the human airway epithelial cell transcriptome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A number of studies have shown that cigarette smoking produces a field defect, such that genetic mutations induced by smoking occur throughout the lung and its intra and extra-pulmonary airways. Based on this concept, we have begun this study, which has as its goal the definition of the normal airway transcriptome, an analysis of how that transcriptome is affected by cigarette smoke, and to explore the reversibility of altered gene expression when smoking has been discontinued. We have obtained brushings from intra-pulmonary airways (the right upper lobe carina) and scrapings from the buccal mucosa, from normal smoking and non-smoking volunteers (including 34 Current Smokers, 23 Never Smokers and 18 Former Smokers). RNA was isolated from these samples and gene expression profiles from intra-pulmonary airway epithelial cells were analyzed using Affymetrix U133A human gene expression arrays. All microarray data from the experiments described above have been stored, preprocessed and analyzed in a relational MySQL database that is accessible through our website at http://pulm.bumc.bu.edu/aged
Keywords = Lung
Keywords = Cancer
Keywords = Airway
Keywords = Epithelium
Keywords = Transcriptome
Keywords = Gene
Keywords = Microarray
Keywords = Bronchi
Keywords: repeat sample
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| Contributor(s) |
Spira A, Beane J, Shah V, Liu G, Schembri F, Yang X, Palma J, Brody J |
| Citation(s) |
15210990 |
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| Submission date |
Jan 25, 2004 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Avrum Spira |
| E-mail(s) |
[email protected]
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| Phone |
617-638-4860
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| Fax |
617-536-8093
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| URL |
http://www.pulmonomics.com
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| Organization name |
Boston University Medical Center
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| Department |
Pulmonary and Critical Care Medicine
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| Lab |
Pulmonomics Lab
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| Street address |
715 Albany Street, R304
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (75)
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| Relations |
| BioProject |
PRJNA88045 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE994_RAW.tar |
238.4 Mb |
(http)(custom) |
TAR (of CEL) |
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