GEO DataSets

(Submitter supplied) We previously constructed a congenic mouse, B6.S-D2Mit194-D2Mit311 (B6.S-2) with SPRET/Ei donor DNA on distal chromosome 2 in a C57BL/6J background that captured an obesity quantitative trait locus (QTL). Mice homozygous for SPRET/Ei alleles at the donor region had decreased body weight and obesity related phenotypes. The B6.S-2 congenic donor region spanned a minimum of 26 Mb. In this study, we constructed five overlapping subcongenics with smaller SPRET/Ei donor regions to fine map the underlying gene(s). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4479

24 Samples

Download data: TXT

(Submitter supplied) A C57BL6/J (B6) x CAST/Ei (CAST) strain intercross was used to identify the first mammalian QTL for macronutrient-specific intake (carbohydrate and fat) and for total energy intake. A region on proximal Chromosome 17 revealed two significant QTL that co-localized for increased macronutrient intake-carbohydrate (Mnic1) and total kilocalorie intake (Kcal2), adjusted for body weight. An interval-specific congenic strain, B6.CAST-17, was then developed which verified the QTL. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

12 Samples

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(Submitter supplied) We identified a congenic mouse with an introgressed region from the A/J donor inbred strain on an inbred C57BL/6J background that showed a reduced locomotor stimulant response to methamphetamine. We conducted microarray analysis of the striatum from drug-naive female and male mice that were 6-9 weeks old. The congenic region is on chromosome 11 and spans approximately 84-96 Mb. There were two groups of mice used in the analysis: B6 control mice versus congenic mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

28 Samples

Download data: CEL, TXT

(Submitter supplied) The goal of this experiment was to identify genes contributing to fat, carbohydrate, and total calorie intake in mice by comparing gene expression in liver and hypothalamus of an interval-specific congenic strain, B6.CAST-17, with that of the control strain. This congenic strain has previously confirmed genetic loci on Chr 17 linked to increased carbohydrate (Mnic1) and kilocalorie (Kcal2) intake. Keywords: Comparative gene expression analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

8 Samples

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(Submitter supplied) SMXA-5 mouse shows a high-fat induced fatty liver. The QTL for fatty liver was mapped on mouse chromosome 12, designated as Fl1sa. The liver weight and liver triglyceride content of A/J-12SM consomic mouse was lower than that of A/J mouse. In contrast, the epididymal fat weight tended to be higher in A/J-12SM consomic mouse than that in A/J. We compared the epididymal fat's gene expressions between A/J mouse and A/J-12SM consomic mouse.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

2 Samples

Download data: CEL, CHP

(Submitter supplied) SMXA-5 mouse shows a high-fat induced fatty liver. The QTL for fatty liver was mapped on mouse chromosome 12, designated as Fl1sa. The liver triglycerides content of A/J-12SM consomic mouse, a chromosome substitution strain, was suppressed compared to that of A/J mouse. We compared the hepatic gene expressions between A/J mouse and A/J-12SM consomic mouse.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) The specific genes influencing the quantitative variation in macronutrient preference and food intake are virtually unknown. We refined a previously identified mouse chromosome 17 (MMU17) region harboring quantitative trait loci (QTL) with large effects on preferential macronutrient intake-carbohydrate (Mnic1), total kilcalories (Kcal2), and total food volume (Tfv1) using interval-specific congenic strains. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15907

24 Samples

Download data: TXT

(Submitter supplied) We used Affymetrix Mouse Exon 1.0 ST arrays to identify potential changes in alternative splicing between a subcongenic interval of the QTL Sicd2 on Chr 15 and their FVB-like littermates. Results implicate novel candidate genes conferring susceptibility to seizure-induced cell death.

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6193 GPL6096

32 Samples

Download data: CEL

(Submitter supplied) Several studies have shown that bone mineral density (BMD), a clinically measurable predictor of osteoporotic fracture, is the sum of genetic and environmental influences. In addition, serum IGF-1 levels have been correlated to both BMD and fracture risk. We previously identified a Quantitative Trait Locus (QTL) for Bone Mineral Density (BMD) on mouse Chromosome (Chr) 6 that overlaps a QTL for serum IGF-1. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2442

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of livers of animals with decreased serum insulin-like growth factor-I (IGF-I) levels. The liver is the major source of serum IGF-I. The level of serum IGF-I is correlated to bone mineral density (BMD) and fracture risk. Results provide insight into the relationship between IGF-I and BMD.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 other, 2 strain sets

Platform:

GPL1261

Series:

GSE5959

6 Samples

Download data: CEL

(Submitter supplied) Liver and BAT expression differences between the fat F-line, and congenic Fob3b-line. Normalised data appended as follows: TABLE 1: LIVER - 1st set (7.5K) of the 2 slide NIA NIH 15K set -source1 = F-line liver male - 2 pools each made from 5 different individuals -source2 = Fob3b-line (aka Fchr15D-line) liver male - 2 pools each made from 5 different individuals -values are normalised using mixed model across 6 slides: 1 F-line vs Fline 2 Fob3b-line vs Fob3b-line 3 F-line pool1 vs Fob3b-line pool1 4 F-line pool1 vs Fob3b-line pool1 -dye swap 5 F-line pool2 vs Fob3b-line pool2 6 F-line pool2 vs Fob3b-line pool2 -dye swap TABLE 2: LIVER - 2nd set (7.5K) of the 2 slide NIA NIH 15K set -source1 = F-line liver male - 2 pools each made from 5 differnt individuals -source2 = Fob3b-line (aka Fchr15D-line) liver male - 2 pools each made from 5 differnt individuals -values are normalised using mixed model across 6 slides: 1 F-line Vs F-line 2 Fob3b-line vs Fob3b-line 3 F-line pool1 vs Fob3b-line pool2 4 F-line pool1 vs Fob3b-line pool2 dye swap 5 F-line pool2 vs Fob3b-line pool1 6 F-line pool2 vs Fob3b-line pool1 dye swap TABLE 3: Brown Adipose Tissue (BAT) 1st (7.5K) array set of 15K NIA NIH set -source1 = F-line male BAT from 2 pools each made from 5 different individuals -source2 = Fob3b-line (aka Fchr15D-line) male BAT from 2 pools each made from 5 different individuals -values are normalised using mixed model across 4 slides: 1 F-line pool1 vs Fob3b-line pool1 2 F-line pool1 vs Fob3b-line pool1 dye swap 3 F-line pool2 vs Fob3b-line pool2 4 F-line pool2 vs Fob3b-line pool2 dye swap TABLE 4: Brown Adipose Tissue (BAT) 2nd (7.5K) array of the 15K NIA NIH set -source1 = F-line male BAT (2 pools each from 5 individual mice) -source2 = Fob3b-line (aka Fchr15D-line) male BAT (2 pools each from 5 individual mice) -values are normalised using mixed model across 4 slides: 1 F-line pool1 vs Fob3b-line pool1 2 F-line pool1 vs Fob3b-line pool1 dye swap 3 F-line pool2 vs Fob3b-line pool2 4 F-line pool2 vs Fob3b-line pool2 dye swap Keywords = Obesity Keywords = Fob3b Keywords = QTL Keywords = congenic Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1341 GPL1342

20 Samples

Download data

(Submitter supplied) The first goal of the study was to find the genes that explain the difference in progression of age-related retinal degeneration (ageRD) between the inbred strains C57BL/6J-c2J (B6a) and BALB/cByJ (C). The specific goal was to find the candidate genes that explain the chromosome 6 and chromosome 10 quantitative trait loci (QTL) found in a previous study. Genes differentially expressed in retina between both strains and that map to the QTL are candidate genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL9214

16 Samples

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(Submitter supplied) These data are from the brains (amygdala and hippocampus) of mice originally derived from a cross between C57BL/6J and A/J inbred strains. We used short-term selection to produce outbred mouse lines with differences in contextual fear conditioning, which is a measure of fear learning. We selected for a total of 4 generations. Fear learning differed in the selected lines and this difference was stronger with each successive generation of selection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1901

Platform:

GPL1261

24 Samples

Download data: CEL

Analysis of the amygdalae and hippocampi of strains exhibiting low or high freezing behavior in response to fear. Previous work identified alleles that contribute to the variability in freezing behavior, and the chromosomal location of these alleles.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 tissue sets

Platform:

GPL1261

Series:

GSE4034

24 Samples

Download data: CEL

(Submitter supplied) A congenic line was constructed by introgressing a C3H chromosome 1 region harboring Bglu3 into C57BL/6 apoE-/- background. RNA was extracted from liver using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the liver of Bglu3 congenic mice and C57BL/6 apoE-deficient mouse strains fed a western diet.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL

(Submitter supplied) Abstract: Interleukin-10-deficient (Il10-/-) mice serve as a model for inflammatory bowel disease (IBD). The severity of colitis strongly depends on the inbred strain carrying the disrupted Il10 gene: C3H/HeJBir (C3) confers disease susceptibility, whereas C57BL/6J (B6) confers resistance. Genome-wide scans with microsatellite markers on segregrating backcross and F2 populations resulted in the detection of ten colitogenic quantitative trait loci (QTL). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1871

Platform:

GPL81

8 Samples

Download data: CEL, EXP

Analysis of colons of C3H/HeJBir and C57BL/6J strains following interleukin-10 (IL-10) gene disruption. IL-10 disruption leads to colitis in C3H/HeJBir strain but not in C57BL/6J. Results used to reduce the number of candidate genes associated with colitogenic quantitative trait loci regions.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 strain sets

Platform:

GPL81

Series:

GSE2172

8 Samples

Download data: CEL, EXP

(Submitter supplied) Background and Aims: In the interleukin-10-deficient (Il10-/-) mouse model of IBD, 10 quantitative trait loci (QTL) have been shown to be associated with colitis susceptibility by linkage analyses on experimental crosses of highly susceptible C3H/HeJBir (C3Bir)-Il10-/- and partially resistant C57BL/6J (B6)-Il10-/- mice. The strongest locus (C3Bir-derived cytokine deficiency-induced colitis susceptibility [Cdcs]1 on Chromosome [Chr] 3) controlled multiple colitogenic subphenotypes and contributed the vast majority to the phenotypic variance in cecum and colon. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

24 Samples

Download data: CEL

(Submitter supplied) Pooled RNA samples were prepared for each of the four mouse strainsDU6, DU6i, DUKs, and DBA by epididymal fat tissues of 42 days old 20 male per lines. Pooling was used to reduce individual variability, which is high in outbred populations. For the hybridization of the tissue specific RNAs to the GeneChips, samples were prepared according to the recommendations of the Affymetrix user guide. First strand synthesis was carried out by a T7-(dT)24 primer and SuperScript II Reverse Transcriptase (Gibco BRL, Life Technologies GmbH, Eggenstein, Germany) using 10 mg whole RNA sample. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL76 GPL75

8 Samples

Download data

(Submitter supplied) To identify novel mechanisms regulating allogeneic hematopoietic cell engraftment, we previously used a forward genetic approach and described identification, in mice, of the Bmgr5 bone marrow (BM) engraftment quantitative trait locus (QTL). This QTL confers dominant and large allele effects for engraftment susceptibility. It was localized to chromosome 16 by classical quantitative genetic techniques in a segregating backcross bred from susceptible BALB.K and resistant B10.BR mice. more...

Organism:

Mus musculus

Type:

Genome variation profiling by SNP array

Platform:

GPL13147

2 Samples

Download data: CEL