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Links from GEO DataSets

Items: 20

1.

Human blastocyst model by self-organization of naïve pluripotent stem cells

(Submitter supplied) Human naïve pluripotent cells can differentiate to extraembryonic trophoblast and hypoblast cells. Here we report formation of human blastocyst models by self-organization solely of naïve pluripotent stem cells. The embryo models comprise the three founding lineages, epiblast, trophoblast and hypoblast, arranged to mimic the natural blastocyst. Single-cell RNA sequencing validated the identity of each cell type.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
495 Samples
Download data: CSV, TXT
Series
Accession:
GSE171820
ID:
200171820
2.

Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential II

(Submitter supplied) Transcriptomic analysis confirms the trophoblast identity of the naïve pluripotent stem cell derived TSC cultures by comparing to the placenta derived TSC culture.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
14 Samples
Download data: TXT
3.

Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential

(Submitter supplied) Classical mouse embryology has established a paradigm of early development drivenby sequential lineage bifurcations. Accordingly, mouse embryonic stem cells derivedfrom early epiblast have lost the potency to produce extraembryonic trophectoderm.We show in contrast that human naive epiblast cells readily make trophectoderm.Inhibition of ERK signalling, instrumental in naive stem cell propagation, unexpectedlypotentiates trophectoderm formation, an effect enhanced by Nodal inhibition.Transcriptome analyses authenticate conversion into trophectoderm with subsequentproduction of syncitiotrophoblast, cytotrophoblast and trophoblast stem cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
20 Samples
Download data: TXT
Series
Accession:
GSE166422
ID:
200166422
4.

Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential

(Submitter supplied) Classical mouse embryology has established a paradigm of early development drivenby sequential lineage bifurcations. Accordingly, mouse embryonic stem cells derivedfrom early epiblast have lost the potency to produce extraembryonic trophectoderm.We show in contrast that human naive epiblast cells readily make trophectoderm.Inhibition of ERK signalling, instrumental in naive stem cell propagation, unexpectedlypotentiates trophectoderm formation, an effect enhanced by Nodal inhibition.Transcriptome analyses authenticate conversion into trophectoderm with subsequentproduction of syncitiotrophoblast, cytotrophoblast and trophoblast stem cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
64 Samples
Download data: TXT
5.

Human blastoids model blastocyst development and implantation [Bulk RNA-seq]

(Submitter supplied) Shortly after fertilization, human embryos implant into the uterus. This requires the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Appropriate stem cells can form a blastocyst model, which we termed blastoid. Here we show that naive human pluripotent stem cells (hPSCs) triply inhibited for the Hippo, TGF-β, and ERK pathways consistently and efficiently (>70%) form blastoids that generate transcriptional pre-implantation analogs of the three founding lineages (trophoblast, epiblast, primitive endoderm; >97%) according to the  sequence and pace of blastocyst development. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
61 Samples
Download data: TXT
6.

Human blastoids model blastocyst development and implantation

(Submitter supplied) About one week after fertilization, human embryos implant into the uterus. This necessitates the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model, which we termed blastoid. Here we show that naive human pluripotent stem cells (PXGL hPSCs) efficiently (>70%) form blastoids generating blastocyst-stage analogs of the 3 founding lineages (>97% trophectoderm, epiblast, and primitive endoderm) according to the sequence and to the pace of blastocyst development. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
2776 Samples
Download data: RDS, TSV, TXT
Series
Accession:
GSE177689
ID:
200177689
7.

Human blastoids model blastocyst development and implantation [scRNAseq]

(Submitter supplied) Shortly after fertilization, human embryos implant into the uterus. This requires the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form blastocyst models, which we termed blastoid. Here we show that naïve human pluripotent stem cells (hPSCs) triply inhibited for the Hippo, TGFb, and ERK pathways consistently and efficiently (>70%) form blastoids that generate transcriptional pre-implantation (>95%) analogs of the three founding lineages (trophoblast, epiblast, hypoblast), and in the sequential and timely manner of blastocysts. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
2715 Samples
Download data: TXT
Series
Accession:
GSE177616
ID:
200177616
8.

Single Cell RNA-seq analysis of gene expression patterns of cells from stem-cell-derived-synthetic-blastocysts, called as 'ES-' or 'EPS-blastoids'.

(Submitter supplied) To test the efficacy of stem cell lines to generate 'stem-cell-derived-synthetic blastocysts', we dissociated ES- (built by conventional embryonic stem cells and trophoblast stem cells) or EPS-blastoids (built by extended potential pluripotent stem cells and trophoblast stem cells) into single cells following 96h of culture for single-cell transcriptome analysis.
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
1 Sample
Download data: MTX, TSV, TXT
Series
Accession:
GSE134240
ID:
200134240
9.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
9 Samples
Download data: TAB
Series
Accession:
GSE200935
ID:
200200935
10.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates [scRNA-seq]

(Submitter supplied) Our study provides an alternative strategy to generate human blastoids and model early embryogenesis up to pre-gastrulation stage, facilitating the insights into human embryonic development
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
1 Sample
Download data: TAB
Series
Accession:
GSE200934
ID:
200200934
11.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates [RNA-seq]

(Submitter supplied) Our study provides an alternative strategy to generate human blastoids and model early embryogenesis up to pre-gastrulation stage, facilitating the insights into human embryonic development
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
8 Samples
Download data: TXT
Series
Accession:
GSE200933
ID:
200200933
12.

Derivation of trophoblast stem cells from naïve human pluripotent stem cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL21290 GPL18573
22 Samples
Download data: BIGWIG
Series
Accession:
GSE138762
ID:
200138762
13.

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

(Submitter supplied) Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. more...
Organism:
Homo sapiens
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL18573
6 Samples
Download data: BIGWIG
Series
Accession:
GSE138761
ID:
200138761
14.

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

(Submitter supplied) Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21290
20 Samples
Download data: TXT
15.

TFAP2C regulates transcription in human naive pluripotency by opening enhancers

(Submitter supplied) Naive and primed pluripotent human embryonic stem cells bear transcriptional similarity to pre- and post-implantation epiblast and thus constitute a developmental model for understanding the pluripotent stages in human embryo development. To identify new transcription factors that differentially regulate the unique pluripotent stages, we mapped open chromatin using ATAC-seq and found enrichment of the activator protein-2 (AP2) transcription factor binding motif at naive-specific open chromatin. more...
Organism:
Homo sapiens; Mus musculus
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
4 related Platforms
109 Samples
Download data: BW, TXT
Series
Accession:
GSE101074
ID:
200101074
16.

Generation of blastocyst-like structures from human embryonic stem cells III [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
4 Samples
Download data: CSV
Series
Accession:
GSE163445
ID:
200163445
17.

Generation of blastocyst-like structures from human embryonic stem cells II [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
4 Samples
Download data: CSV
Series
Accession:
GSE163423
ID:
200163423
18.

Generation of blastocyst-like structures from human embryonic stem cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
9 Samples
Download data: CSV
Series
Accession:
GSE150578
ID:
200150578
19.

Generation of blastocyst-like structures from human embryonic stem cells I [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
1 Sample
Download data: CSV
Series
Accession:
GSE150570
ID:
200150570
20.

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells (single-cell RNA-Seq)

(Submitter supplied) A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
2 Samples
Download data: MTX, TSV
Series
Accession:
GSE135701
ID:
200135701
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