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Links from GEO DataSets

Items: 20

1.

Human blastoids model blastocyst development and implantation [scRNAseq]

(Submitter supplied) Shortly after fertilization, human embryos implant into the uterus. This requires the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form blastocyst models, which we termed blastoid. Here we show that naïve human pluripotent stem cells (hPSCs) triply inhibited for the Hippo, TGFb, and ERK pathways consistently and efficiently (>70%) form blastoids that generate transcriptional pre-implantation (>95%) analogs of the three founding lineages (trophoblast, epiblast, hypoblast), and in the sequential and timely manner of blastocysts. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
2715 Samples
Download data: TXT
Series
Accession:
GSE177616
ID:
200177616
2.

Human blastoids model blastocyst development and implantation [Bulk RNA-seq]

(Submitter supplied) Shortly after fertilization, human embryos implant into the uterus. This requires the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Appropriate stem cells can form a blastocyst model, which we termed blastoid. Here we show that naive human pluripotent stem cells (hPSCs) triply inhibited for the Hippo, TGF-β, and ERK pathways consistently and efficiently (>70%) form blastoids that generate transcriptional pre-implantation analogs of the three founding lineages (trophoblast, epiblast, primitive endoderm; >97%) according to the  sequence and pace of blastocyst development. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
61 Samples
Download data: TXT
3.

Human blastoids model blastocyst development and implantation

(Submitter supplied) About one week after fertilization, human embryos implant into the uterus. This necessitates the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model, which we termed blastoid. Here we show that naive human pluripotent stem cells (PXGL hPSCs) efficiently (>70%) form blastoids generating blastocyst-stage analogs of the 3 founding lineages (>97% trophectoderm, epiblast, and primitive endoderm) according to the sequence and to the pace of blastocyst development. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
2776 Samples
Download data: RDS, TSV, TXT
Series
Accession:
GSE177689
ID:
200177689
4.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
9 Samples
Download data: TAB
Series
Accession:
GSE200935
ID:
200200935
5.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates [scRNA-seq]

(Submitter supplied) Our study provides an alternative strategy to generate human blastoids and model early embryogenesis up to pre-gastrulation stage, facilitating the insights into human embryonic development
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
1 Sample
Download data: TAB
Series
Accession:
GSE200934
ID:
200200934
6.

Modelling human pre-gastulation development by 3D culture of blastoids generated from the primed-to-naive transitioning intermediates [RNA-seq]

(Submitter supplied) Our study provides an alternative strategy to generate human blastoids and model early embryogenesis up to pre-gastrulation stage, facilitating the insights into human embryonic development
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
8 Samples
Download data: TXT
Series
Accession:
GSE200933
ID:
200200933
7.

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells (single-cell RNA-Seq)

(Submitter supplied) A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
2 Samples
Download data: MTX, TSV
Series
Accession:
GSE135701
ID:
200135701
8.

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells

(Submitter supplied) A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21273
8 Samples
Download data: TXT
Series
Accession:
GSE135289
ID:
200135289
9.

Generation of blastocyst-like structures from human embryonic stem cells III [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
4 Samples
Download data: CSV
Series
Accession:
GSE163445
ID:
200163445
10.

Generation of blastocyst-like structures from human embryonic stem cells II [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
4 Samples
Download data: CSV
Series
Accession:
GSE163423
ID:
200163423
11.

Generation of blastocyst-like structures from human embryonic stem cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
9 Samples
Download data: CSV
Series
Accession:
GSE150578
ID:
200150578
12.

Generation of blastocyst-like structures from human embryonic stem cells I [scRNA-seq]

(Submitter supplied) We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
1 Sample
Download data: CSV
Series
Accession:
GSE150570
ID:
200150570
13.

Highly efficient generation of blastocyst-like structures from mouse totipotent blastomere-like cells

(Submitter supplied) Mammalian embryogenesis begins with a totipotent zygote. Early embryogenesis can be recapitulated by aggregated extended pluripotent stem cells (EPSCs) in a 3D culture system. However, the efficiency of generating blastoids is low and whether other reported totipotent stem cells retained a similar capacity remains unknown. Here we show that spliceosomal repressed totipotent blastomere-like cells (TBLCs) form blastocyst-like structures when aggregated in 3D microwells with around 80% efficiency. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL17021 GPL24247
8 Samples
Download data: TXT
Series
Accession:
GSE197779
ID:
200197779
14.

Human blastocyst model by self-organization of naïve pluripotent stem cells

(Submitter supplied) Human naïve pluripotent cells can differentiate to extraembryonic trophoblast and hypoblast cells. Here we report formation of human blastocyst models by self-organization solely of naïve pluripotent stem cells. The embryo models comprise the three founding lineages, epiblast, trophoblast and hypoblast, arranged to mimic the natural blastocyst. Single-cell RNA sequencing validated the identity of each cell type.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
495 Samples
Download data: CSV, TXT
Series
Accession:
GSE171820
ID:
200171820
15.

Single Cell RNA-seq analysis of gene expression patterns of cells from stem-cell-derived-synthetic-blastocysts, called as 'ES-' or 'EPS-blastoids'.

(Submitter supplied) To test the efficacy of stem cell lines to generate 'stem-cell-derived-synthetic blastocysts', we dissociated ES- (built by conventional embryonic stem cells and trophoblast stem cells) or EPS-blastoids (built by extended potential pluripotent stem cells and trophoblast stem cells) into single cells following 96h of culture for single-cell transcriptome analysis.
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
1 Sample
Download data: MTX, TSV, TXT
Series
Accession:
GSE134240
ID:
200134240
16.

Blastoidverse

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
1 Sample
Download data: CSV
Series
Accession:
GSE210962
ID:
200210962
17.

Human blastoid

(Submitter supplied) Based on our previous works, we established an optimized protocol that enables the generation of blastocyst-like structures from human pluripotent stem cells.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
1 Sample
Download data: CSV
Series
Accession:
GSE210961
ID:
200210961
18.

Self-renewing human naïve pluripotent stem cells dedifferentiate in 3D culture and form blastoids spontaneously

(Submitter supplied) Human naïve pluripotent stem cells (hnPSCs) can generate integrated models of blastocysts termed blastoids upon switch to inductive medium. However, the underlying mechanisms remain obscure. Here we report that self-renewing hnPSCs spontaneously and efficiently give rise to blastoids upon three dimensional (3D) suspension culture. The spontaneous blastoids mimic early stage human blastocysts in terms of structure, size, and transcriptome characteristics and are capable of progressing to post-implantation stages. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL30209 GPL24676
164 Samples
Download data: CSV, MTX, TSV
Series
Accession:
GSE252114
ID:
200252114
19.

Generation of human blastocyst-like structures from pluripotent stem cells

(Submitter supplied) Due to ethical concerns and restricted access to human blastocysts, we lack a comprehensive understanding of early human embryogenesis. A reliable model system that can recapitulate early stages of human embryogenesis would help solve this problem.Here we report a robust three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
3 Samples
Download data: MTX, TSV
Series
Accession:
GSE158971
ID:
200158971
20.

The chemically-defined induction of a primitive endoderm and epiblast-like niche supports post-implantation progression from blastoids.

(Submitter supplied) The early mammalian conceptus (blastocyst) contains two supporting extraembryonic tissues - the trophectoderm and the primitive endoderm (PrE) - that encase and guide the epiblast (Epi) to eventually form the all body. Modifications of the conceptus exposed key genes regulating these tissues co-development. However, the combination of signalling pathways at play remains elusive. Stem cell-based models including embryoid bodies and blastoids can be generated in large numbers and subjected to high-content screens. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
1955 Samples
Download data: TSV
Series
Accession:
GSE129655
ID:
200129655
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